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| [[Image:114d.gif|left|200px]]<br /><applet load="114d" size="450" color="white" frame="true" align="right" spinBox="true"
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| caption="114d, resolution 2.500Å" />
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| '''INOSINE-ADENINE BASE PAIRS IN A B-DNA DUPLEX'''<br />
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| ==Overview== | | ==INOSINE-ADENINE BASE PAIRS IN A B-DNA DUPLEX== |
| The structure of the synthetic deoxydodecamer d(C-G-C-I-A-A-T-T-A-G-C-G), has been determined by single crystal X-ray diffraction techniques at 2.5A, resolution. The refinement converged with a crystallographic residual, R =, 0.19 and the location of 64 solvent molecules. The sequence crystallises, as a B-DNA helix with 10 Watson-Crick base-pairs (4 A.T. and 6 G.C) and 2, inosine.adenine (I.A) pairs. The present work shows that in the, purine.purine base-pairs the adenine adopts syn orientation with respect, to the furanose moiety while the inosine is in the trans (anti), orientation. Two hydrogen bonds link the I.A. base-pair, one between, N-1(I) and N-7(A), the other between O-6(I) and N-6(A). This bulky, purine.purine base-pair is incorporated in the double helix at two, positions with little distortion of either local or global conformation., The pairing observed in this study is presented as a model for I.A, base-pairs in RNA codon-anticodon interactions and may help explain the, thermodynamic stability of inosine containing base-pairs. Conformational, parameters and base stacking interactions are presented and where, appropriate compared with those of the native compound, d(C-G-C-G-A-A-T-T-C-G-C-G) and with other studies of oligonucleotides, containing purine.purine base-pairs.
| | <StructureSection load='114d' size='340' side='right'caption='[[114d]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[114d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=114D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=114D FirstGlance]. <br> |
| 114D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=114D OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=114d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=114d OCA], [https://pdbe.org/114d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=114d RCSB], [https://www.ebi.ac.uk/pdbsum/114d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=114d ProSAT]</span></td></tr> |
| ==Reference== | | </table> |
| Inosine.adenine base pairs in a B-DNA duplex., Corfield PW, Hunter WN, Brown T, Robinson P, Kennard O, Nucleic Acids Res. 1987 Oct 12;15(19):7935-49. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=3671069 3671069]
| | __TOC__ |
| [[Category: Protein complex]]
| | </StructureSection> |
| [[Category: Brown, T.]] | | [[Category: Large Structures]] |
| [[Category: Corfield, P.W.R.]]
| | [[Category: Brown T]] |
| [[Category: Hunter, W.N.]]
| | [[Category: Corfield PWR]] |
| [[Category: Kennard, O.]] | | [[Category: Hunter WN]] |
| [[Category: Robinson, P.]] | | [[Category: Kennard O]] |
| [[Category: b-dna]] | | [[Category: Robinson P]] |
| [[Category: double helix]] | |
| [[Category: mismatched]] | |
| [[Category: modified]] | |
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| ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 21:45:51 2007''
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