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| [[Image:114d.gif|left|200px]]
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| {{Structure
| | ==INOSINE-ADENINE BASE PAIRS IN A B-DNA DUPLEX== |
| |PDB= 114d |SIZE=350|CAPTION= <scene name='initialview01'>114d</scene>, resolution 2.500Å
| | <StructureSection load='114d' size='340' side='right'caption='[[114d]], [[Resolution|resolution]] 2.50Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DI:2'-DEOXYINOSINE-5'-MONOPHOSPHATE'>DI</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>
| | <table><tr><td colspan='2'>[[114d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=114D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=114D FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| |GENE=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=114d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=114d OCA], [https://pdbe.org/114d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=114d RCSB], [https://www.ebi.ac.uk/pdbsum/114d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=114d ProSAT]</span></td></tr> |
| |DOMAIN=
| | </table> |
| |RELATEDENTRY=
| | __TOC__ |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=114d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=114d OCA], [http://www.ebi.ac.uk/pdbsum/114d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=114d RCSB]</span>
| | </StructureSection> |
| }}
| | [[Category: Large Structures]] |
| | | [[Category: Brown T]] |
| '''INOSINE-ADENINE BASE PAIRS IN A B-DNA DUPLEX'''
| | [[Category: Corfield PWR]] |
| | | [[Category: Hunter WN]] |
| | | [[Category: Kennard O]] |
| ==Overview==
| | [[Category: Robinson P]] |
| The structure of the synthetic deoxydodecamer d(C-G-C-I-A-A-T-T-A-G-C-G) has been determined by single crystal X-ray diffraction techniques at 2.5A resolution. The refinement converged with a crystallographic residual, R = 0.19 and the location of 64 solvent molecules. The sequence crystallises as a B-DNA helix with 10 Watson-Crick base-pairs (4 A.T. and 6 G.C) and 2 inosine.adenine (I.A) pairs. The present work shows that in the purine.purine base-pairs the adenine adopts syn orientation with respect to the furanose moiety while the inosine is in the trans (anti) orientation. Two hydrogen bonds link the I.A. base-pair, one between N-1(I) and N-7(A), the other between O-6(I) and N-6(A). This bulky purine.purine base-pair is incorporated in the double helix at two positions with little distortion of either local or global conformation. The pairing observed in this study is presented as a model for I.A base-pairs in RNA codon-anticodon interactions and may help explain the thermodynamic stability of inosine containing base-pairs. Conformational parameters and base stacking interactions are presented and where appropriate compared with those of the native compound, d(C-G-C-G-A-A-T-T-C-G-C-G) and with other studies of oligonucleotides containing purine.purine base-pairs.
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| ==About this Structure==
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| 114D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=114D OCA].
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| ==Reference==
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| Inosine.adenine base pairs in a B-DNA duplex., Corfield PW, Hunter WN, Brown T, Robinson P, Kennard O, Nucleic Acids Res. 1987 Oct 12;15(19):7935-49. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3671069 3671069]
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| [[Category: Protein complex]] | |
| [[Category: Brown, T.]] | |
| [[Category: Corfield, P W.R.]] | |
| [[Category: Hunter, W N.]] | |
| [[Category: Kennard, O.]] | |
| [[Category: Robinson, P.]] | |
| [[Category: b-dna]]
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| [[Category: double helix]]
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| [[Category: mismatched]]
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| [[Category: modified]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:26:51 2008''
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