1r9u: Difference between revisions
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==Refined structure of peptaibol zervamicin IIB in methanol solution from trans-hydrogen bond J couplings== | ==Refined structure of peptaibol zervamicin IIB in methanol solution from trans-hydrogen bond J couplings== | ||
<StructureSection load='1r9u' size='340' side='right'caption='[[1r9u | <StructureSection load='1r9u' size='340' side='right'caption='[[1r9u]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1r9u]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[1r9u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Emericellopsis_salmosynnemata Emericellopsis salmosynnemata]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R9U FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=AIB:ALPHA-AMINOISOBUTYRIC+ACID'>AIB</scene>, <scene name='pdbligand=DIV:D-ISOVALINE'>DIV</scene>, <scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene>, <scene name='pdbligand=PHL:L-PHENYLALANINOL'>PHL</scene>, <scene name='pdbligand=PRD_000159:Zervamicin+IIB'>PRD_000159</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9u OCA], [https://pdbe.org/1r9u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r9u RCSB], [https://www.ebi.ac.uk/pdbsum/1r9u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r9u ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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[[Category: Emericellopsis salmosynnemata]] | [[Category: Emericellopsis salmosynnemata]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Arseniev | [[Category: Arseniev AS]] | ||
[[Category: Balashova | [[Category: Balashova TA]] | ||
[[Category: Ovchinnikova | [[Category: Ovchinnikova TV]] | ||
[[Category: Shenkarev | [[Category: Shenkarev ZO]] | ||
[[Category: Yakimenko | [[Category: Yakimenko ZA]] | ||
Latest revision as of 16:58, 1 February 2024
Refined structure of peptaibol zervamicin IIB in methanol solution from trans-hydrogen bond J couplingsRefined structure of peptaibol zervamicin IIB in methanol solution from trans-hydrogen bond J couplings
Structural highlights
Publication Abstract from PubMedZervamicin IIB is a member of the alpha-aminoisobutyric acid containing peptaibol antibiotics. A new procedure for the biosynthetic preparation of the uniformly 13C- and 15N-enriched peptaibol is described This compound was isolated from the biomass of the fungus-producer Emericellopsis salmosynnemata strain 336 IMI 58330 obtained upon cultivation in the totally 13C, 15N-labelled complete medium. To prepare such a medium the autolysed biomass and the exopolysaccharides of the obligate methylotrophic bacterium Methylobacillus flagellatus KT were used. This microorganism was grown in totally 13C, 15N-labelled minimal medium containing 13C-methanol and 15N-ammonium chloride as the only carbon and nitrogen sources. Preliminary NMR spectroscopic analysis indicated a high extent of isotope incorporation (> 90%) and led to the complete 13C- and 15N-NMR assignment including the stereospecific assignment of Aib residues methyl groups. The observed pattern of the structurally important secondary chemical shifts of 1H(alpha), 13C=O and 13C(alpha) agrees well with the previously determined structure of zervamicin IIB in methanol solution. Biosynthetic uniform 13C,15N-labelling of zervamicin IIB. Complete 13C and 15N NMR assignment.,Ovchinnikova TV, Shenkarev ZO, Yakimenko ZA, Svishcheva NV, Tagaev AA, Skladnev DA, Arseniev AS J Pept Sci. 2003 Nov-Dec;9(11-12):817-26. PMID:14658801[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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