7osm: Difference between revisions

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-'''Unreleased structure'''
-The entry 7osm is ON HOLD
+==Intermediate translocation complex of 80 S.cerevisiae ribosome with eEF2 and ligands==
+<StructureSection load='7osm' size='340' side='right'caption='[[7osm]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7osm]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OSM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OSM FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=YG:WYBUTOSINE'>YG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7osm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7osm OCA], [https://pdbe.org/7osm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7osm RCSB], [https://www.ebi.ac.uk/pdbsum/7osm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7osm ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RL8A_YEAST RL8A_YEAST]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Translation of the genetic code into proteins is realized through repetitions of synchronous translocation of messenger RNA (mRNA) and transfer RNAs (tRNA) through the ribosome. In eukaryotes translocation is ensured by elongation factor 2 (eEF2), which catalyses the process and actively contributes to its accuracy(1). Although numerous studies point to critical roles for both the conserved eukaryotic posttranslational modification diphthamide in eEF2 and tRNA modifications in supporting the accuracy of translocation, detailed molecular mechanisms describing their specific functions are poorly understood. Here we report a high-resolution X-ray structure of the eukaryotic 80S ribosome in a translocation-intermediate state containing mRNA, naturally modified eEF2 and tRNAs. The crystal structure reveals a network of stabilization of codon-anticodon interactions involving diphthamide(1) and the hypermodified nucleoside wybutosine at position 37 of phenylalanine tRNA, which is also known to enhance translation accuracy(2). The model demonstrates how the decoding centre releases a codon-anticodon duplex, allowing its movement on the ribosome, and emphasizes the function of eEF2 as a 'pawl' defining the directionality of translocation(3). This model suggests how eukaryote-specific elements of the 80S ribosome, eEF2 and tRNAs undergo large-scale molecular reorganizations to ensure maintenance of the mRNA reading frame during the complex process of translocation.
-Authors: Djumagulov, M., Jenner, L., Rozov, A., Demeshkina, N., Yusupov, M., Yusupova, G.
+Accuracy mechanism of eukaryotic ribosome translocation.,Djumagulov M, Demeshkina N, Jenner L, Rozov A, Yusupov M, Yusupova G Nature. 2021 Dec;600(7889):543-546. doi: 10.1038/s41586-021-04131-9. Epub 2021 , Dec 1. PMID:34853469<ref>PMID:34853469</ref>
-Description: Intermediate translocation complex of 80 S.cerevisiae ribosome with eEF2 and ligands
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Rozov, A]]
+<div class="pdbe-citations 7osm" style="background-color:#fffaf0;"></div>
-[[Category: Jenner, L]]
-[[Category: Yusupova, G]]
+==See Also==
-[[Category: Yusupov, M]]
+*[[Elongation factor 3D structures|Elongation factor 3D structures]]
-[[Category: Demeshkina, N]]
+*[[Ribosome 3D structures|Ribosome 3D structures]]
-[[Category: Djumagulov, M]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Saccharomyces cerevisiae]]
+[[Category: Demeshkina N]]
+[[Category: Djumagulov M]]
+[[Category: Jenner L]]
+[[Category: Rozov A]]
+[[Category: Yusupov M]]
+[[Category: Yusupova G]]