7a99: Difference between revisions

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-'''Unreleased structure'''
-The entry 7a99 is ON HOLD  until Paper Publication
+==Crystal structure of the Phe57Trp mutant of the arginine-bound form of domain 1 from TmArgBP==
+<StructureSection load='7a99' size='340' side='right'caption='[[7a99]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7a99]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] and [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A99 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A99 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.79&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ARG:ARGININE'>ARG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a99 OCA], [https://pdbe.org/7a99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a99 RCSB], [https://www.ebi.ac.uk/pdbsum/7a99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a99 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9WZ62_THEMA Q9WZ62_THEMA]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Arginine is one of the most important nutrients of living organisms as it plays a major role in important biological pathways. However, the accumulation of arginine as consequence of metabolic defects causes hyperargininemia, an autosomal recessive disorder. Therefore, the efficient detection of the arginine is a field of relevant biomedical/biotechnological interest. Here, we developed protein variants suitable for arginine sensing by mutating and dissecting the multimeric and multidomain structure of Thermotoga maritima arginine-binding protein (TmArgBP). Indeed, previous studies have shown that TmArgBP domain-swapped structure can be manipulated to generate simplified monomeric and single domain scaffolds. On both these stable scaffolds, to measure tryptophan fluorescence variations associated with the arginine binding, a Phe residue of the ligand binding pocket was mutated to Trp. Upon arginine binding, both mutants displayed a clear variation of the Trp fluorescence. Notably, the single domain scaffold variant exhibited a good affinity (~3 microM) for the ligand. Moreover, the arginine binding to this variant could be easily reverted under very mild conditions. Atomic-level data on the recognition process between the scaffold and the arginine were obtained through the determination of the crystal structure of the adduct. Collectively, present data indicate that TmArgBP scaffolds represent promising candidates for developing arginine biosensors.
-Authors: Balasco, N., Vitagliano, L., Smaldone, G., Ruggiero, A.
+Development of a Protein Scaffold for Arginine Sensing Generated through the Dissection of the Arginine-Binding Protein from Thermotoga maritima.,Smaldone G, Ruggiero A, Balasco N, Vitagliano L Int J Mol Sci. 2020 Oct 12;21(20). pii: ijms21207503. doi: 10.3390/ijms21207503. PMID:33053818<ref>PMID:33053818</ref>
-Description: Crystal structure of the Phe57Trp mutant of the arginine-bound form of domain 1 from TmArgBP
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Balasco, N]]
+<div class="pdbe-citations 7a99" style="background-color:#fffaf0;"></div>
-[[Category: Smaldone, G]]
-[[Category: Ruggiero, A]]
+==See Also==
-[[Category: Vitagliano, L]]
+*[[ABC transporter 3D structures|ABC transporter 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Thermotoga maritima]]
+[[Category: Thermotoga maritima MSB8]]
+[[Category: Balasco N]]
+[[Category: Ruggiero A]]
+[[Category: Smaldone G]]
+[[Category: Vitagliano L]]