6zzg: Difference between revisions
New page: '''Unreleased structure''' The entry 6zzg is ON HOLD Authors: Hatzopoulos, G.N., Kukenshoner, T., Banterle, N., Favez, T., Fluckiger, I., Hantschel, O., Gonczy, P. Description: MB_CRS6... |
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==MB_CRS6-1 bound to CrSAS-6_N== | |||
<StructureSection load='6zzg' size='340' side='right'caption='[[6zzg]], [[Resolution|resolution]] 2.93Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6zzg]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZZG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZZG FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.93Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zzg OCA], [https://pdbe.org/6zzg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zzg RCSB], [https://www.ebi.ac.uk/pdbsum/6zzg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zzg ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A9CQL4_CHLRE A9CQL4_CHLRE] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Centrioles are evolutionarily conserved multi-protein organelles essential for forming cilia and centrosomes. Centriole biogenesis begins with self-assembly of SAS-6 proteins into 9-fold symmetrical ring polymers, which then stack into a cartwheel that scaffolds organelle formation. The importance of this architecture has been difficult to decipher notably because of the lack of precise tools to modulate the underlying assembly reaction. Here, we developed monobodies against Chlamydomonas reinhardtii SAS-6, characterizing three in detail with X-ray crystallography, atomic force microscopy and cryo-electron microscopy. This revealed distinct monobody-target interaction modes, as well as specific consequences on ring assembly and stacking. Of particular interest, monobody MB(CRS6)-15 induces a conformational change in CrSAS-6, resulting in the formation of a helix instead of a ring. Furthermore, we show that this alteration impairs centriole biogenesis in human cells. Overall, our findings identify monobodies as powerful molecular levers to alter the architecture of multi-protein complexes and tune centriole assembly. | |||
Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly.,Hatzopoulos GN, Kukenshoner T, Banterle N, Favez T, Fluckiger I, Hamel V, Andany S, Fantner GE, Hantschel O, Gonczy P Nat Commun. 2021 Jun 21;12(1):3805. doi: 10.1038/s41467-021-23897-0. PMID:34155202<ref>PMID:34155202</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6zzg" style="background-color:#fffaf0;"></div> | ||
[[Category: Banterle | == References == | ||
[[Category: | <references/> | ||
[[Category: Gonczy | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Chlamydomonas reinhardtii]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Mus musculus]] | |||
[[Category: Banterle N]] | |||
[[Category: Favez T]] | |||
[[Category: Fluckiger I]] | |||
[[Category: Gonczy P]] | |||
[[Category: Hantschel O]] | |||
[[Category: Hatzopoulos GN]] | |||
[[Category: Kukenshoner T]] | |||