6zyj: Difference between revisions

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-'''Unreleased structure'''
-The entry 6zyj is ON HOLD  until Paper Publication
+==Crystal structure of Hsc70 ATPase domain in complex with ADP and calcium==
+<StructureSection load='6zyj' size='340' side='right'caption='[[6zyj]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6zyj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZYJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZYJ FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zyj OCA], [https://pdbe.org/6zyj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zyj RCSB], [https://www.ebi.ac.uk/pdbsum/6zyj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zyj ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/HSP7C_HUMAN HSP7C_HUMAN] Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex.<ref>PMID:10722728</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The metazoan endoplasmic reticulum (ER) serves both as a hub for maturation of secreted proteins and as an intracellular calcium storage compartment, facilitating calcium release-dependent cellular processes. ER calcium depletion robustly activates the unfolded protein response (UPR). However, it is unclear how fluctuations in ER calcium impact organellar proteostasis. Here we report that calcium selectively affects the dynamics of the abundant metazoan ER Hsp70 chaperone BiP, by enhancing its affinity for ADP. In the calcium-replete ER, ADP rebinding to post-ATP hydrolysis BiP-substrate complexes competes with ATP binding during both spontaneous and co-chaperone-assisted nucleotide exchange, favouring substrate retention. Conversely, in the calcium-depleted ER, relative acceleration of ADP-to-ATP exchange favours substrate release. These findings explain the rapid dissociation of certain substrates from BiP observed in the calcium-depleted ER and suggest a mechanism for tuning ER quality control and coupling UPR activity to signals that mobilise ER calcium in secretory cells.
-Authors: Yan, Y., Preissler, S., Ron, D.
+Calcium depletion challenges endoplasmic reticulum proteostasis by destabilising BiP-substrate complexes.,Preissler S, Rato C, Yan Y, Perera LA, Czako A, Ron D Elife. 2020 Dec 9;9. pii: 62601. doi: 10.7554/eLife.62601. PMID:33295873<ref>PMID:33295873</ref>
-Description: Crystal structure of Hsc70 ATPase domain in complex with ADP and calcium
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Yan, Y]]
+<div class="pdbe-citations 6zyj" style="background-color:#fffaf0;"></div>
-[[Category: Ron, D]]
-[[Category: Preissler, S]]
+==See Also==
+*[[Heat Shock Protein structures|Heat Shock Protein structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Preissler S]]
+[[Category: Ron D]]
+[[Category: Yan Y]]