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==K97A MUTANT OF E9 DNASE DOMAIN IN COMPLEX WITH IM9==
==K97A mutant of E9 DNase domain in complex with Im9==
<StructureSection load='2vlo' size='340' side='right' caption='[[2vlo]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='2vlo' size='340' side='right'caption='[[2vlo]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2vlo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VLO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VLO FirstGlance]. <br>
<table><tr><td colspan='2'>[[2vlo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VLO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VLO FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ivz|2ivz]], [[1v14|1v14]], [[1fsj|1fsj]], [[1v15|1v15]], [[1bxi|1bxi]], [[1imp|1imp]], [[1imq|1imq]], [[1v13|1v13]], [[1e0h|1e0h]], [[1emv|1emv]], [[1fr2|1fr2]], [[2vln|2vln]], [[2vlq|2vlq]], [[2vlp|2vlp]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vlo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vlo OCA], [http://pdbe.org/2vlo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vlo RCSB], [http://www.ebi.ac.uk/pdbsum/2vlo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vlo ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vlo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vlo OCA], [https://pdbe.org/2vlo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vlo RCSB], [https://www.ebi.ac.uk/pdbsum/2vlo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vlo ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/IMM9_ECOLX IMM9_ECOLX]] This protein is able to protect a cell, which harbors the plasmid ColE9 encoding colicin E9, against colicin E9, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. [[http://www.uniprot.org/uniprot/CEA9_ECOLX CEA9_ECOLX]] This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity.  Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.  
[https://www.uniprot.org/uniprot/IMM9_ECOLX IMM9_ECOLX] This protein is able to protect a cell, which harbors the plasmid ColE9 encoding colicin E9, against colicin E9, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vl/2vlo_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vl/2vlo_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
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==See Also==
==See Also==
*[[Colicin|Colicin]]
*[[Colicin 3D structures|Colicin 3D structures]]
*[[Colicin Immunity Protein|Colicin Immunity Protein]]
*[[Colicin immunity protein 3D structures|Colicin immunity protein 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bacillus coli migula 1895]]
[[Category: Escherichia coli]]
[[Category: Baker, D]]
[[Category: Large Structures]]
[[Category: Joachimiak, L A]]
[[Category: Baker D]]
[[Category: Keeble, A H]]
[[Category: Joachimiak LA]]
[[Category: Kirkpatrick, N]]
[[Category: Keeble AH]]
[[Category: Kleanthous, C]]
[[Category: Kirkpatrick N]]
[[Category: Mate, M J]]
[[Category: Kleanthous C]]
[[Category: Meenan, N]]
[[Category: Mate MJ]]
[[Category: Antibiotic]]
[[Category: Meenan N]]
[[Category: Antimicrobial]]
[[Category: Bacteriocin]]
[[Category: Bacteriocin immunity]]
[[Category: Colicin]]
[[Category: Endonuclease]]
[[Category: Hth motif]]
[[Category: Hydrolase]]
[[Category: Metal-binding]]
[[Category: Nuclease]]
[[Category: Protein-binding]]
[[Category: Protein-protein interaction]]

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