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==Crystal structure of Aeromonas exotoxin A== | ==Crystal structure of Aeromonas exotoxin A== | ||
<StructureSection load='6z5h' size='340' side='right'caption='[[6z5h]]' scene=''> | <StructureSection load='6z5h' size='340' side='right'caption='[[6z5h]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z5H OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[6z5h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeromonas_hydrophila Aeromonas hydrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z5H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z5H FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z5h OCA], [https://pdbe.org/6z5h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z5h RCSB], [https://www.ebi.ac.uk/pdbsum/6z5h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z5h ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Aeromonas exotoxin A (AE) is a bacterial virulence factor recently discovered in a clinical case of necrotising fasciitis caused by the flesh-eating Aeromonas hydrophila. Here, database mining shows that AE is present in the genome of several emerging Aeromonas pathogenic species. The X-ray crystal structure of AE was solved at 2.3 A and presents all the hallmarks common to diphthamide-specific mono-ADP-ribosylating toxins, suggesting AE is a fourth member of this family alongside the diphtheria toxin, Pseudomonas exotoxin A and cholix. Structural homology indicates AE may use a similar mechanism of cytotoxicity that targets eukaryotic elongation factor 2 and thus inhibition of protein synthesis. The structure of AE also highlights unique features including a metal binding site, and a negatively charged cleft that could play a role in interdomain interactions and may affect toxicity. This study raises new opportunities to engineer alternative toxin-based molecules with pharmaceutical potential. | |||
Crystal Structure of Exotoxin A from Aeromonas Pathogenic Species.,Masuyer G Toxins (Basel). 2020 Jun 15;12(6). pii: toxins12060397. doi:, 10.3390/toxins12060397. PMID:32549399<ref>PMID:32549399</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6z5h" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Exotoxin 3D structures|Exotoxin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Aeromonas hydrophila]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Masuyer G]] | [[Category: Masuyer G]] | ||
Latest revision as of 16:42, 24 January 2024
Crystal structure of Aeromonas exotoxin ACrystal structure of Aeromonas exotoxin A
Structural highlights
Publication Abstract from PubMedAeromonas exotoxin A (AE) is a bacterial virulence factor recently discovered in a clinical case of necrotising fasciitis caused by the flesh-eating Aeromonas hydrophila. Here, database mining shows that AE is present in the genome of several emerging Aeromonas pathogenic species. The X-ray crystal structure of AE was solved at 2.3 A and presents all the hallmarks common to diphthamide-specific mono-ADP-ribosylating toxins, suggesting AE is a fourth member of this family alongside the diphtheria toxin, Pseudomonas exotoxin A and cholix. Structural homology indicates AE may use a similar mechanism of cytotoxicity that targets eukaryotic elongation factor 2 and thus inhibition of protein synthesis. The structure of AE also highlights unique features including a metal binding site, and a negatively charged cleft that could play a role in interdomain interactions and may affect toxicity. This study raises new opportunities to engineer alternative toxin-based molecules with pharmaceutical potential. Crystal Structure of Exotoxin A from Aeromonas Pathogenic Species.,Masuyer G Toxins (Basel). 2020 Jun 15;12(6). pii: toxins12060397. doi:, 10.3390/toxins12060397. PMID:32549399[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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