6y33: Difference between revisions

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OCA

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-'''Unreleased structure'''
-The entry 6y33 is ON HOLD
+==Streptavidin mutant S112R with a biotC5-1 cofactor - an artificial iron hydroxylase==
+<StructureSection load='6y33' size='340' side='right'caption='[[6y33]], [[Resolution|resolution]] 1.49&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6y33]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y33 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Y33 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.49&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=O7Q:biotC5-1+cofactor'>O7Q</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6y33 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y33 OCA], [https://pdbe.org/6y33 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6y33 RCSB], [https://www.ebi.ac.uk/pdbsum/6y33 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6y33 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The selective hydroxylation of C-H bonds is of great interest to the synthetic community. Both homogeneous catalysts and enzymes offer complementary means to tackle this challenge. Herein, we show that biotinylated Fe(TAML)-complexes (TAML = Tetra Amido Macrocyclic Ligand) can be used as cofactors for incorporation into streptavidin to assemble artificial hydroxylases. Chemo-genetic optimization of both cofactor and streptavidin allowed optimizing the performance of the hydroxylase. Using H2O2 as oxidant, up to approximately 300 turnovers for the oxidation of benzylic C-H bonds were obtained. Upgrading the ee was achieved by kinetic resolution of the resulting benzylic alcohol to afford up to &gt;98% ee for (R)-tetralol. X-ray analysis of artificial hydroxylases highlights critical details of the second coordination sphere around the Fe(TAML) cofactor.
-Authors:
+Enantioselective Hydroxylation of Benzylic C(sp(3))-H Bonds by an Artificial Iron Hydroxylase Based on the Biotin-Streptavidin Technology.,Serrano-Plana J, Rumo C, Rebelein JG, Peterson RL, Barnet M, Ward TR J Am Chem Soc. 2020 Jun 17;142(24):10617-10623. doi: 10.1021/jacs.0c02788. Epub, 2020 Jun 3. PMID:32450689<ref>PMID:32450689</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6y33" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Avidin 3D structures|Avidin 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Streptomyces avidinii]]
+[[Category: Barnet M]]
+[[Category: Peterson RL]]
+[[Category: Rebelein JG]]
+[[Category: Rumo C]]
+[[Category: Serrano-Plana J]]
+[[Category: Ward TR]]