6t1u: Difference between revisions
New page: '''Unreleased structure''' The entry 6t1u is ON HOLD Authors: Opperman, D.J., Sewell, B.T. Description: Cytochrome P450 reductase from Candida tropicalis [[Category: Unreleased Structu... |
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==Cytochrome P450 reductase from Candida tropicalis== | |||
<StructureSection load='6t1u' size='340' side='right'caption='[[6t1u]], [[Resolution|resolution]] 1.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6t1u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_tropicalis Candida tropicalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T1U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6T1U FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6t1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t1u OCA], [https://pdbe.org/6t1u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6t1u RCSB], [https://www.ebi.ac.uk/pdbsum/6t1u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6t1u ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q5PXH3_CANTR Q5PXH3_CANTR] This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis.[HAMAP-Rule:MF_03212] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Cytochrome P450 reductases (CPRs) are diflavin oxidoreductases that supply electrons to type II cytochrome P450 monooxygenases (CYPs). In addition, it can also reduce other proteins and molecules, including cytochrome c, ferricyanide, and different drugs. Although various CPRs have been functionally and structurally characterized, the overall mechanism and its interaction with different redox acceptors remain elusive. One of the main problems regarding electron transfer between CPRs and CYPs is the so-called "uncoupling", whereby NAD(P)H derived electrons are lost due to the reduced intermediates' (FAD and FMN of CPR) interaction with molecular oxygen. Additionally, the decay of the iron-oxygen complex of the CYP can also contribute to loss of reducing equivalents during an unproductive reaction cycle. This phenomenon generates reactive oxygen species (ROS), leading to an inefficient reaction. Here, we present the study of the CPR from Candida tropicalis (CtCPR) lacking the hydrophobic N-terminal part (Delta2-22). The enzyme supports the reduction of cytochrome c and ferricyanide, with an estimated 30% uncoupling during the reactions with cytochrome c. The ROS produced was not influenced by different physicochemical conditions (ionic strength, pH, temperature). The X-ray structures of the enzyme were solved with and without its cofactor, NADPH. Both CtCPR structures exhibited the closed conformation. Comparison with the different solved structures revealed an intricate ionic network responsible for the regulation of the open/closed movement of CtCPR. | |||
Biochemical and structural insights into the cytochrome P450 reductase from Candida tropicalis.,Ebrecht AC, van der Bergh N, Harrison STL, Smit MS, Sewell BT, Opperman DJ Sci Rep. 2019 Dec 27;9(1):20088. doi: 10.1038/s41598-019-56516-6. PMID:31882753<ref>PMID:31882753</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6t1u" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
==See Also== | |||
*[[NADPH-Cytochrome P450 Reductase|NADPH-Cytochrome P450 Reductase]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Candida tropicalis]] | |||
[[Category: Large Structures]] | |||
[[Category: Opperman DJ]] | |||
[[Category: Sewell BT]] | |||
Latest revision as of 15:52, 24 January 2024
Cytochrome P450 reductase from Candida tropicalisCytochrome P450 reductase from Candida tropicalis
Structural highlights
FunctionQ5PXH3_CANTR This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis.[HAMAP-Rule:MF_03212] Publication Abstract from PubMedCytochrome P450 reductases (CPRs) are diflavin oxidoreductases that supply electrons to type II cytochrome P450 monooxygenases (CYPs). In addition, it can also reduce other proteins and molecules, including cytochrome c, ferricyanide, and different drugs. Although various CPRs have been functionally and structurally characterized, the overall mechanism and its interaction with different redox acceptors remain elusive. One of the main problems regarding electron transfer between CPRs and CYPs is the so-called "uncoupling", whereby NAD(P)H derived electrons are lost due to the reduced intermediates' (FAD and FMN of CPR) interaction with molecular oxygen. Additionally, the decay of the iron-oxygen complex of the CYP can also contribute to loss of reducing equivalents during an unproductive reaction cycle. This phenomenon generates reactive oxygen species (ROS), leading to an inefficient reaction. Here, we present the study of the CPR from Candida tropicalis (CtCPR) lacking the hydrophobic N-terminal part (Delta2-22). The enzyme supports the reduction of cytochrome c and ferricyanide, with an estimated 30% uncoupling during the reactions with cytochrome c. The ROS produced was not influenced by different physicochemical conditions (ionic strength, pH, temperature). The X-ray structures of the enzyme were solved with and without its cofactor, NADPH. Both CtCPR structures exhibited the closed conformation. Comparison with the different solved structures revealed an intricate ionic network responsible for the regulation of the open/closed movement of CtCPR. Biochemical and structural insights into the cytochrome P450 reductase from Candida tropicalis.,Ebrecht AC, van der Bergh N, Harrison STL, Smit MS, Sewell BT, Opperman DJ Sci Rep. 2019 Dec 27;9(1):20088. doi: 10.1038/s41598-019-56516-6. PMID:31882753[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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