6rw3: Difference between revisions
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<StructureSection load='6rw3' size='340' side='right'caption='[[6rw3]], [[Resolution|resolution]] 3.65Å' scene=''> | <StructureSection load='6rw3' size='340' side='right'caption='[[6rw3]], [[Resolution|resolution]] 3.65Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6rw3]] is a 4 chain structure with sequence from [ | <table><tr><td colspan='2'>[[6rw3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RW3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RW3 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.65Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rw3 OCA], [https://pdbe.org/6rw3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rw3 RCSB], [https://www.ebi.ac.uk/pdbsum/6rw3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rw3 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/O97467_PLAFA O97467_PLAFA] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Plasmodium falciparum]] | ||
[[Category: Brock | [[Category: Brock J]] | ||
[[Category: Drew | [[Category: Drew D]] | ||
[[Category: Matsuoka | [[Category: Matsuoka R]] | ||
[[Category: Qureshi | [[Category: Qureshi A]] | ||
Latest revision as of 15:29, 24 January 2024
The molecular basis for sugar import in malaria parasites.The molecular basis for sugar import in malaria parasites.
Structural highlights
FunctionPublication Abstract from PubMedElucidating the mechanism of sugar import requires a molecular understanding of how transporters couple sugar binding and gating events. Whereas mammalian glucose transporters (GLUTs) are specialists(1), the hexose transporter from the malaria parasite Plasmodium falciparum PfHT1(2,3) has acquired the ability to transport both glucose and fructose sugars as efficiently as the dedicated glucose (GLUT3) and fructose (GLUT5) transporters. Here, to establish the molecular basis of sugar promiscuity in malaria parasites, we determined the crystal structure of PfHT1 in complex with D-glucose at a resolution of 3.6 A. We found that the sugar-binding site in PfHT1 is very similar to those of the distantly related GLUT3 and GLUT5 structures(4,5). Nevertheless, engineered PfHT1 mutations made to match GLUT sugar-binding sites did not shift sugar preferences. The extracellular substrate-gating helix TM7b in PfHT1 was positioned in a fully occluded conformation, providing a unique glimpse into how sugar binding and gating are coupled. We determined that polar contacts between TM7b and TM1 (located about 15 A from D-glucose) are just as critical for transport as the residues that directly coordinate D-glucose, which demonstrates a strong allosteric coupling between sugar binding and gating. We conclude that PfHT1 has achieved substrate promiscuity not by modifying its sugar-binding site, but instead by evolving substrate-gating dynamics. The molecular basis for sugar import in malaria parasites.,Qureshi AA, Suades A, Matsuoka R, Brock J, McComas SE, Nji E, Orellana L, Claesson M, Delemotte L, Drew D Nature. 2020 Feb;578(7794):321-325. doi: 10.1038/s41586-020-1963-z. Epub 2020 Jan, 29. PMID:31996846[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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