6r2v: Difference between revisions
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==== | ==Arabidopsis NF-Y/CCAAT-box complex== | ||
<StructureSection load='6r2v' size='340' side='right'caption='[[6r2v]]' scene=''> | <StructureSection load='6r2v' size='340' side='right'caption='[[6r2v]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[6r2v]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R2V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6R2V FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6r2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r2v OCA], [https://pdbe.org/6r2v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6r2v RCSB], [https://www.ebi.ac.uk/pdbsum/6r2v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6r2v ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.503Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6r2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r2v OCA], [https://pdbe.org/6r2v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6r2v RCSB], [https://www.ebi.ac.uk/pdbsum/6r2v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6r2v ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/NFYA6_ARATH NFYA6_ARATH] Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
NF-Y transcription factor comprises three subunits: NF-YA, NF-YB and NF-YC. NF-YB and NF-YC dimerize through their histone fold domain (HFD), which can bind DNA in a non-sequence-specific fashion while serving as a scaffold for NF-YA trimerization. Upon trimerization, NF-YA specifically recognizes the CCAAT box sequence on promoters and enhancers. In plants, each NF-Y subunit is encoded by several genes giving rise to hundreds of potential heterotrimeric combinations. In addition, plant NF-YBs and NF-YCs interact with other protein partners to recognize a plethora of genomic motifs, as the CCT protein family that binds CORE sites. The NF-Y subunit organization and its DNA-binding properties, together with the NF-Y HFD capacity to adapt different protein modules, represent plant-specific features that play a key role in development, growth and reproduction. Despite their relevance, these features are still poorly understood at the molecular level. Here, we present the structures of Arabidopsis and rice NF-YB/NF-YC dimers, and of an Arabidopsis NF-Y trimer in complex with the FT CCAAT box, together with biochemical data on NF-Y mutants. The dimeric structures identify the key residues for NF-Y HFD stabilization. The NF-Y/DNA structure and the mutation experiments shed light on HFD trimerization interface properties and the NF-YA sequence appetite for the bases flanking the CCAAT motif. These data explain the logic of plant NF-Y gene expansion: the trimerization adaptability and the flexible DNA-binding rules serve the scopes of accommodating the large number of NF-YAs, CCTs and possibly other NF-Y HFD binding partners and a diverse audience of genomic motifs. | |||
Structural determinants for NF-Y subunit organization and NF-Y/DNA association in plants.,Chaves-Sanjuan A, Gnesutta N, Gobbini A, Martignago D, Bernardini A, Fornara F, Mantovani R, Nardini M Plant J. 2021 Jan;105(1):49-61. doi: 10.1111/tpj.15038. Epub 2020 Nov 27. PMID:33098724<ref>PMID:33098724</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6r2v" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Nuclear transcription factor Y|Nuclear transcription factor Y]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Arabidopsis thaliana]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Bernardini A]] | ||
[[Category: Chaves-Sanjuan A]] | |||
[[Category: Chiara M]] | |||
[[Category: Fornara F]] | |||
[[Category: Gnesutta N]] | |||
[[Category: Horner D]] | |||
[[Category: Mantovani R]] | |||
[[Category: Nardini M]] | |||