6qvv: Difference between revisions
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<StructureSection load='6qvv' size='340' side='right'caption='[[6qvv]], [[Resolution|resolution]] 2.40Å' scene=''> | <StructureSection load='6qvv' size='340' side='right'caption='[[6qvv]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6qvv]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[6qvv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Toscana_virus Toscana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QVV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QVV FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN3:MANGANESE+(III)+ION'>MN3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN3:MANGANESE+(III)+ION'>MN3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qvv OCA], [https://pdbe.org/6qvv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qvv RCSB], [https://www.ebi.ac.uk/pdbsum/6qvv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qvv ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/L_TOSV L_TOSV] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 6qvv" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 6qvv" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Toscana virus]] | ||
[[Category: Bragagniolo | [[Category: Bragagniolo G]] | ||
[[Category: Jones | [[Category: Jones R]] | ||
[[Category: Lessoued | [[Category: Lessoued S]] | ||
[[Category: Mate | [[Category: Mate M]] | ||
[[Category: Reguera | [[Category: Reguera J]] | ||
Latest revision as of 15:09, 24 January 2024
Structure and function of phenuiviridae cap snatching endonucleasesStructure and function of phenuiviridae cap snatching endonucleases
Structural highlights
FunctionPublication Abstract from PubMedToscana virus (TOSV) is an arthropod-borne human pathogen responsible for seasonal outbreaks of fever and meningoencephalitis in the Mediterranean basin. TOSV is a segmented negative-strand RNA virus (sNSV) that belongs to the genus phlebovirus (family Phenuiviridae, order Bunyavirales), encompassing other important human pathogens such as Rift Valley fever virus (RVFV). Here, we carried out a structural and functional characterization of the TOSV cap-snatching endonuclease, an N terminal domain of the viral polymerase (L protein) that provides capped 3'OH primers for transcription. We report TOSV endonuclease crystal structures in the apo form, in complex with a di-ketoacid inhibitor (DPBA) and in an intermediate state of inhibitor release, showing details on substrate binding and active site dynamics. The structure reveals substantial folding rearrangements absent in previously reported cap-snatching endonucleases. These include the relocation of the N terminus and the appearance of new structural motifs important for transcription and replication. The enzyme shows high activity rates comparable to other His+ cap-snatching endonucleases. Moreover, the activity is dependent on conserved residues involved in metal ion and substrate binding. Altogether, these results bring new light on the structure and function of cap-snatching endonucleases and pave the way for the development of specific and broad-spectrum antivirals. Structure and function of the Toscana virus cap-snatching endonuclease.,Jones R, Lessoued S, Meier K, Devignot S, Barata-Garcia S, Mate M, Bragagnolo G, Weber F, Rosenthal M, Reguera J Nucleic Acids Res. 2019 Oct 4. pii: 5580917. doi: 10.1093/nar/gkz838. PMID:31584100[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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