6i6y: Difference between revisions
New page: '''Unreleased structure''' The entry 6i6y is ON HOLD Authors: Wang, H., Logan, D.T., Oliveberg, M. Description: Circular permutant of ribosomal protein S6, swap helix 2 [[Category: Unr... |
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The | ==Circular permutant of ribosomal protein S6, swap helix 2== | ||
<StructureSection load='6i6y' size='340' side='right'caption='[[6i6y]], [[Resolution|resolution]] 2.15Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6i6y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I6Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6I6Y FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6i6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i6y OCA], [https://pdbe.org/6i6y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6i6y RCSB], [https://www.ebi.ac.uk/pdbsum/6i6y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6i6y ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RS6_THET8 RS6_THET8] Located on the outer edge of the platform on the body of the 30S subunit.[HAMAP-Rule:MF_00360] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Although folded proteins are commonly depicted as simplistic combinations of beta-strands and alpha-helices, the actual properties and functions of these secondary-structure elements in their native contexts are just partly understood. The principal reason is that the behavior of individual beta- and alpha-elements is obscured by the global folding cooperativity. In this study, we have circumvented this problem by designing frustrated variants of the mixed alpha/beta-protein S6, which allow the structural behavior of individual beta-strands and alpha-helices to be targeted selectively by stopped-flow kinetics, X-ray crystallography, and solution-state NMR. Essentially, our approach is based on provoking intramolecular "domain swap." The results show that the alpha- and beta-elements have quite different characteristics: The swaps of beta-strands proceed via global unfolding, whereas the alpha-helices are free to swap locally in the native basin. Moreover, the alpha-helices tend to hybridize and to promote protein association by gliding over to neighboring molecules. This difference in structural behavior follows directly from hydrogen-bonding restrictions and suggests that the protein secondary structure defines not only tertiary geometry, but also maintains control in function and structural evolution. Finally, our alternative approach to protein folding and native-state dynamics presents a generally applicable strategy for in silico design of protein models that are computationally testable in the microsecond-millisecond regime. | |||
Exposing the distinctive modular behavior of beta-strands and alpha-helices in folded proteins.,Wang H, Logan DT, Danielsson J, Oliveberg M Proc Natl Acad Sci U S A. 2020 Nov 17;117(46):28775-28783. doi:, 10.1073/pnas.1920455117. Epub 2020 Nov 4. PMID:33148805<ref>PMID:33148805</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Logan | <div class="pdbe-citations 6i6y" style="background-color:#fffaf0;"></div> | ||
[[Category: Oliveberg | |||
[[Category: Wang | ==See Also== | ||
*[[Ribosomal protein S6|Ribosomal protein S6]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Thermus thermophilus HB8]] | |||
[[Category: Logan DT]] | |||
[[Category: Oliveberg M]] | |||
[[Category: Wang H]] | |||