6hys: Difference between revisions

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-'''Unreleased structure'''
-The entry 6hys is ON HOLD
+==Crystal structure of DHX8 helicase domain bound to ADP at 2.6 angstrom==
+<StructureSection load='6hys' size='340' side='right'caption='[[6hys]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6hys]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HYS FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hys OCA], [https://pdbe.org/6hys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hys RCSB], [https://www.ebi.ac.uk/pdbsum/6hys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hys ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/DHX8_HUMAN DHX8_HUMAN] Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.<ref>PMID:8608946</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+DHX8 is a crucial DEAH-box RNA helicase involved in splicing and required for the release of mature mRNA from the spliceosome. Here we report the biochemical characterization of full-length human DHX8 and the catalytically active helicase core DHX8Delta547, alongside crystal structures of DHX8Delta547 bound to ADP and a structure of DHX8Delta547 bound to poly(A)6 single-strand RNA. Our results reveal that DHX8 has an in vitro binding preference for adenine-rich RNA and that RNA binding triggers release of ADP through significant conformational flexibility in the conserved DEAH-, P-loop and hook-turn motifs. We demonstrate the importance of R620 and both the hook-turn and hook-loop regions for DHX8 helicase activity, and propose that the hook-turn acts as a gatekeeper to regulate directional movement of the 3' end of RNA through the RNA binding channel. This study provides an in-depth understanding of the activity of DHX8 and contributes insights into the RNA unwinding mechanisms of the DEAH-box helicase family.
-Authors: Felisberto-Rodrigues, C., Thomas, J.C., McAndrew, P.C., Le Bihan, Y.V., Burke, R., Workman, P., van Montfort, R.L.M.
+Structural and functional characterisation of human RNA helicase DHX8 provides insights into the mechanism of RNA-stimulated ADP release.,Felisberto-Rodrigues C, Thomas JC, McAndrew C, Le Bihan YV, Burke R, Workman P, van Montfort RLM Biochem J. 2019 Aug 13. pii: BCJ20190383. doi: 10.1042/BCJ20190383. PMID:31409651<ref>PMID:31409651</ref>
-Description: Crystal structure of DHX8 helicase domain bound to ADP at 2.6 angstrom
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Burke, R]]
+<div class="pdbe-citations 6hys" style="background-color:#fffaf0;"></div>
-[[Category: Felisberto-Rodrigues, C]]
-[[Category: Van Montfort, R.L.M]]
+==See Also==
-[[Category: Workman, P]]
+*[[Helicase 3D structures|Helicase 3D structures]]
-[[Category: Le Bihan, Y.V]]
+== References ==
-[[Category: Mcandrew, P.C]]
+<references/>
-[[Category: Thomas, J.C]]
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Burke R]]
+[[Category: Felisberto-Rodrigues C]]
+[[Category: Le Bihan YV]]
+[[Category: McAndrew PC]]
+[[Category: Thomas JC]]
+[[Category: Workman P]]
+[[Category: Van Montfort RLM]]