5nl1: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-'''Unreleased structure'''
-The entry 5nl1 is ON HOLD
+==Shigella IpaA-VBS3/TBS in complex with the Talin VBS1 domain 488-512==
+<StructureSection load='5nl1' size='340' side='right'caption='[[5nl1]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5nl1]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NL1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NL1 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nl1 OCA], [https://pdbe.org/5nl1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nl1 RCSB], [https://www.ebi.ac.uk/pdbsum/5nl1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nl1 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TLN1_MOUSE TLN1_MOUSE] Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The Shigella type III effector IpaA contains three binding sites for the focal adhesion protein vinculin (VBSs), which are involved in bacterial invasion of host cells. Here, we report that IpaA VBS3 unexpectedly binds to talin. The 2.5 A resolution crystal structure of IpaA VBS3 in complex with the talin H1-H4 helices shows a tightly folded alpha-helical bundle, which is in contrast to the bundle unraveling upon vinculin interaction. High-affinity binding to talin H1-H4 requires a core of hydrophobic residues and electrostatic interactions conserved in talin VBS H46. Remarkably, IpaA VBS3 localizes to filopodial distal adhesions enriched in talin, but not vinculin. In addition, IpaA VBS3 binding to talin was required for filopodial adhesions and efficient capture of Shigella. These results point to the functional diversity of VBSs and support a specific role for talin binding by a subset of VBSs in the formation of filopodial adhesions.
-Authors:
+Shigella IpaA Binding to Talin Stimulates Filopodial Capture and Cell Adhesion.,Valencia-Gallardo C, Bou-Nader C, Aguilar-Salvador DI, Carayol N, Quenech'Du N, Pecqueur L, Park H, Fontecave M, Izard T, Tran Van Nhieu G Cell Rep. 2019 Jan 22;26(4):921-932.e6. doi: 10.1016/j.celrep.2018.12.091. PMID:30673614<ref>PMID:30673614</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 5nl1" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Talin 3D structures|Talin 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Mus musculus]]
+[[Category: Shigella flexneri]]
+[[Category: Bou-Nader C]]
+[[Category: Fontecave M]]
+[[Category: Pecqueur L]]
+[[Category: Tran Van Nhieu G]]
+[[Category: Valencia-Gallardo C]]