5c9o: Difference between revisions
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==Crystal structure of recombinant PLL lectin from Photorhabdus luminescens at 1.5 A resolution== | |||
<StructureSection load='5c9o' size='340' side='right'caption='[[5c9o]], [[Resolution|resolution]] 1.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5c9o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Photorhabdus_luminescens Photorhabdus luminescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C9O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C9O FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c9o OCA], [https://pdbe.org/5c9o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c9o RCSB], [https://www.ebi.ac.uk/pdbsum/5c9o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c9o ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q7N8J0_PHOLL Q7N8J0_PHOLL] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Photorhabdus luminescens is known for its symbiosis with the entomopathogenic nematode Heterorhabditis bacteriophora and its pathogenicity towards insect larvae. A hypothetical protein from P. luminescens was identified, purified from the native source and characterized as an L-fucose-binding lectin, named PLL. Glycan array and biochemical characterization data revealed PLL to be specific towards L-fucose and the disaccharide glycan 3,6-O-Me2-Glcbeta1-4(2,3-O-Me2)Rhaalpha-O-(p-C6H4)-OCH2CH2NH2. PLL was discovered to be a homo-tetramer with an inter-subunit disulphide bridge. The crystal structures of native (ntPLL) and recombinant PLL (rPLL) revealed a seven-bladed beta-propeller fold creating 7 putative fucose-binding sites per monomer. The crystal structure of the rPLL/L-fucose complex confirmed at least three sites being fucose-binding. Moreover, the crystal structures indicated that some of the other sites are masked either by the tetrameric nature of the lectin or by incorporation of the C-terminus of the lectin into one of these sites. PLL exhibited a binding ability to insect haemocytes and the cuticular surface of a nematode, Heterorhabditis bacteriophora. | |||
A Novel Fucose-Binding Lectin from Photorhabdus luminescens (PLL) with an Unusual Hepta-Bladed beta-Propeller Tetrameric Structure.,Kumar A, Sykorova P, Demo G, Dobes P, Hyrsl P, Wimmerova M J Biol Chem. 2016 Oct 7. pii: jbc.M115.693473. PMID:27758853<ref>PMID:27758853</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5c9o" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: Sykorova | [[Category: Large Structures]] | ||
[[Category: Photorhabdus luminescens]] | |||
[[Category: Demo G]] | |||
[[Category: Dobes P]] | |||
[[Category: Hyrsl P]] | |||
[[Category: Kumar A]] | |||
[[Category: Sykorova P]] | |||
[[Category: Wimmerova M]] | |||
Latest revision as of 14:19, 10 January 2024
Crystal structure of recombinant PLL lectin from Photorhabdus luminescens at 1.5 A resolutionCrystal structure of recombinant PLL lectin from Photorhabdus luminescens at 1.5 A resolution
Structural highlights
FunctionPublication Abstract from PubMedPhotorhabdus luminescens is known for its symbiosis with the entomopathogenic nematode Heterorhabditis bacteriophora and its pathogenicity towards insect larvae. A hypothetical protein from P. luminescens was identified, purified from the native source and characterized as an L-fucose-binding lectin, named PLL. Glycan array and biochemical characterization data revealed PLL to be specific towards L-fucose and the disaccharide glycan 3,6-O-Me2-Glcbeta1-4(2,3-O-Me2)Rhaalpha-O-(p-C6H4)-OCH2CH2NH2. PLL was discovered to be a homo-tetramer with an inter-subunit disulphide bridge. The crystal structures of native (ntPLL) and recombinant PLL (rPLL) revealed a seven-bladed beta-propeller fold creating 7 putative fucose-binding sites per monomer. The crystal structure of the rPLL/L-fucose complex confirmed at least three sites being fucose-binding. Moreover, the crystal structures indicated that some of the other sites are masked either by the tetrameric nature of the lectin or by incorporation of the C-terminus of the lectin into one of these sites. PLL exhibited a binding ability to insect haemocytes and the cuticular surface of a nematode, Heterorhabditis bacteriophora. A Novel Fucose-Binding Lectin from Photorhabdus luminescens (PLL) with an Unusual Hepta-Bladed beta-Propeller Tetrameric Structure.,Kumar A, Sykorova P, Demo G, Dobes P, Hyrsl P, Wimmerova M J Biol Chem. 2016 Oct 7. pii: jbc.M115.693473. PMID:27758853[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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