4y12: Difference between revisions

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-'''Unreleased structure'''
-The entry 4y12 is ON HOLD  until Paper Publication
+==Crystal structure of the S/T protein kinase PknG from Mycobacterium tuberculosis in complex with AGS==
+<StructureSection load='4y12' size='340' side='right'caption='[[4y12]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4y12]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y12 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y12 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y12 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y12 OCA], [https://pdbe.org/4y12 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y12 RCSB], [https://www.ebi.ac.uk/pdbsum/4y12 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y12 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PKNG_MYCTU PKNG_MYCTU] Phosphorylates GarA. May play a role in metabolic regulation via control of the phosphorylation status of GarA. Plays a crucial role in the survival of mycobacteria within host macrophages, by blocking the intracellular degradation of mycobacteria in lysosomes. Required for intrinsic antibiotic resistance.<ref>PMID:19019160</ref> <ref>PMID:19528288</ref> <ref>PMID:19638631</ref> <ref>PMID:17616581</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Tuberculosis remains one of the world's deadliest human diseases, with a high prevalence of antibiotic-resistant Mycobacterium tuberculosis (Mtb) strains. A molecular understanding of processes underlying regulation and adaptation of bacterial physiology may provide novel avenues for the development of antibiotics with unconventional modes of action. Here, we focus on the multidomain S/T protein kinase PknG, a soluble enzyme that controls central metabolism in Actinobacteria and has been linked to Mtb infectivity. Our biochemical and structural studies reveal how different motifs and domains flanking the catalytic core regulate substrate selectivity without significantly affecting the intrinsic kinase activity, whereas a rubredoxin-like domain is shown to downregulate catalysis through specific intramolecular interactions that modulate access to a profound substrate-binding site. Our findings provide the basis for the selective and specific inhibition of PknG, and open new questions about regulation of related bacterial and eukaryotic protein kinases.
-Authors: Lisa, M.N., Alzari, P.M.
+Molecular Basis of the Activity and the Regulation of the Eukaryotic-like S/T Protein Kinase PknG from Mycobacterium tuberculosis.,Lisa MN, Gil M, Andre-Leroux G, Barilone N, Duran R, Biondi RM, Alzari PM Structure. 2015 Apr 29. pii: S0969-2126(15)00126-4. doi:, 10.1016/j.str.2015.04.001. PMID:25960409<ref>PMID:25960409</ref>
-Description: Crystal structure of the S/T protein kinase PknG from Mycobacterium tuberculosis in complex with AGS
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Alzari, P.M]]
+<div class="pdbe-citations 4y12" style="background-color:#fffaf0;"></div>
-[[Category: Lisa, M.N]]
+==See Also==
+*[[Serine/threonine protein kinase 3D structures|Serine/threonine protein kinase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Mycobacterium tuberculosis H37Rv]]
+[[Category: Alzari PM]]
+[[Category: Lisa MN]]