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==Crystal structure of the light-driven sodium pump KR2 in the monomeric blue form, pH 4.3== | ==Crystal structure of the light-driven sodium pump KR2 in the monomeric blue form, pH 4.3== | ||
<StructureSection load='4xtl' size='340' side='right' caption='[[4xtl]], [[Resolution|resolution]] 1.45Å' scene=''> | <StructureSection load='4xtl' size='340' side='right'caption='[[4xtl]], [[Resolution|resolution]] 1.45Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4xtl]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XTL OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[4xtl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dokdonia_eikasta Dokdonia eikasta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XTL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XTL FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=LYR:N~6~-[(2Z,4E,6E,8E)-3,7-DIMETHYL-9-(2,6,6-TRIMETHYLCYCLOHEX-1-EN-1-YL)NONA-2,4,6,8-TETRAENYL]LYSINE'>LYR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xtl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xtl OCA], [https://pdbe.org/4xtl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xtl RCSB], [https://www.ebi.ac.uk/pdbsum/4xtl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xtl ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/N0DKS8_9FLAO N0DKS8_9FLAO] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Recently, the first known light-driven sodium pumps, from the microbial rhodopsin family, were discovered. We have solved the structure of one of them, Krokinobacter eikastus rhodopsin 2 (KR2), in the monomeric blue state and in two pentameric red states, at resolutions of 1.45 A and 2.2 and 2.8 A, respectively. The structures reveal the ion-translocation pathway and show that the sodium ion is bound outside the protein at the oligomerization interface, that the ion-release cavity is capped by a unique N-terminal alpha-helix and that the ion-uptake cavity is unexpectedly large and open to the surface. Obstruction of the cavity with the mutation G263F imparts KR2 with the ability to pump potassium. These results pave the way for the understanding and rational design of cation pumps with new specific properties valuable for optogenetics. | |||
Crystal structure of a light-driven sodium pump.,Gushchin I, Shevchenko V, Polovinkin V, Kovalev K, Alekseev A, Round E, Borshchevskiy V, Balandin T, Popov A, Gensch T, Fahlke C, Bamann C, Willbold D, Buldt G, Bamberg E, Gordeliy V Nat Struct Mol Biol. 2015 Apr 6. doi: 10.1038/nsmb.3002. PMID:25849142<ref>PMID:25849142</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4xtl" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Rhodopsin 3D structures|Rhodopsin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Dokdonia eikasta]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Gordeliy V]] | ||
[[Category: | [[Category: Gushchin I]] | ||
[[Category: | [[Category: Polovinkin V]] | ||
[[Category: | [[Category: Shevchenko V]] | ||
Latest revision as of 13:50, 10 January 2024
Crystal structure of the light-driven sodium pump KR2 in the monomeric blue form, pH 4.3Crystal structure of the light-driven sodium pump KR2 in the monomeric blue form, pH 4.3
Structural highlights
FunctionPublication Abstract from PubMedRecently, the first known light-driven sodium pumps, from the microbial rhodopsin family, were discovered. We have solved the structure of one of them, Krokinobacter eikastus rhodopsin 2 (KR2), in the monomeric blue state and in two pentameric red states, at resolutions of 1.45 A and 2.2 and 2.8 A, respectively. The structures reveal the ion-translocation pathway and show that the sodium ion is bound outside the protein at the oligomerization interface, that the ion-release cavity is capped by a unique N-terminal alpha-helix and that the ion-uptake cavity is unexpectedly large and open to the surface. Obstruction of the cavity with the mutation G263F imparts KR2 with the ability to pump potassium. These results pave the way for the understanding and rational design of cation pumps with new specific properties valuable for optogenetics. Crystal structure of a light-driven sodium pump.,Gushchin I, Shevchenko V, Polovinkin V, Kovalev K, Alekseev A, Round E, Borshchevskiy V, Balandin T, Popov A, Gensch T, Fahlke C, Bamann C, Willbold D, Buldt G, Bamberg E, Gordeliy V Nat Struct Mol Biol. 2015 Apr 6. doi: 10.1038/nsmb.3002. PMID:25849142[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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