4xnj: Difference between revisions
New page: '''Unreleased structure''' The entry 4xnj is ON HOLD Authors: Huang, C.Y., Olieric, V., Diederichs, K., Wang, M., Caffrey, M. Description: X-ray structure of PepTst2 [[Category: Unrele... |
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The | ==X-ray structure of PepTst2== | ||
<StructureSection load='4xnj' size='340' side='right'caption='[[4xnj]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4xnj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_thermophilus_LMG_18311 Streptococcus thermophilus LMG 18311]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XNJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XNJ FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=78M:(2S)-2,3-DIHYDROXYPROPYL(7Z)-PENTADEC-7-ENOATE'>78M</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xnj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xnj OCA], [https://pdbe.org/4xnj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xnj RCSB], [https://www.ebi.ac.uk/pdbsum/4xnj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xnj ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q5M4H8_STRT2 Q5M4H8_STRT2] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The lipid cubic phase (LCP) continues to grow in popularity as a medium in which to generate crystals of membrane (and soluble) proteins for high-resolution X-ray crystallographic structure determination. To date, the PDB includes 227 records attributed to the LCP or in meso method. Among the listings are some of the highest profile membrane proteins, including the beta2-adrenoreceptor-Gs protein complex that figured in the award of the 2012 Nobel Prize in Chemistry to Lefkowitz and Kobilka. The most successful in meso protocol to date uses glass sandwich crystallization plates. Despite their many advantages, glass plates are challenging to harvest crystals from. However, performing in situ X-ray diffraction measurements with these plates is not practical. Here, an alternative approach is described that provides many of the advantages of glass plates and is compatible with high-throughput in situ measurements. The novel in meso in situ serial crystallography (IMISX) method introduced here has been demonstrated with AlgE and PepT (alginate and peptide transporters, respectively) as model integral membrane proteins and with lysozyme as a test soluble protein. Structures were solved by molecular replacement and by experimental phasing using bromine SAD and native sulfur SAD methods to resolutions ranging from 1.8 to 2.8 A using single-digit microgram quantities of protein. That sulfur SAD phasing worked is testament to the exceptional quality of the IMISX diffraction data. The IMISX method is compatible with readily available, inexpensive materials and equipment, is simple to implement and is compatible with high-throughput in situ serial data collection at macromolecular crystallography synchrotron beamlines worldwide. Because of its simplicity and effectiveness, the IMISX approach is likely to supplant existing in meso crystallization protocols. It should prove particularly attractive in the area of ligand screening for drug discovery and development. | |||
In meso in situ serial X-ray crystallography of soluble and membrane proteins.,Huang CY, Olieric V, Ma P, Panepucci E, Diederichs K, Wang M, Caffrey M Acta Crystallogr D Biol Crystallogr. 2015 Jun;71(Pt 6):1238-56. doi:, 10.1107/S1399004715005210. Epub 2015 May 14. PMID:26057665<ref>PMID:26057665</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 4xnj" style="background-color:#fffaf0;"></div> | ||
[[Category: Diederichs | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Symporter 3D structures|Symporter 3D structures]] | ||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Streptococcus thermophilus LMG 18311]] | |||
[[Category: Caffrey M]] | |||
[[Category: Diederichs K]] | |||
[[Category: Huang CY]] | |||
[[Category: Olieric V]] | |||
[[Category: Wang M]] | |||