4wg7: Difference between revisions

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-'''Unreleased structure'''
-The entry 4wg7 is ON HOLD
+==Room-temperature crystal structure of lysozyme determined by serial synchrotron crystallography using a nano focused beam.==
+<StructureSection load='4wg7' size='340' side='right'caption='[[4wg7]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4wg7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WG7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WG7 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wg7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wg7 OCA], [https://pdbe.org/4wg7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wg7 RCSB], [https://www.ebi.ac.uk/pdbsum/4wg7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wg7 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+High-resolution structural information was obtained from lysozyme microcrystals (20 microm in the largest dimension) using raster-scanning serial protein crystallography on micro- and nano-focused beamlines at the ESRF. Data were collected at room temperature (RT) from crystals sandwiched between two silicon nitride wafers, thereby preventing their drying, while limiting background scattering and sample consumption. In order to identify crystal hits, new multi-processing and GUI-driven Python-based pre-analysis software was developed, named NanoPeakCell, that was able to read data from a variety of crystallographic image formats. Further data processing was carried out using CrystFEL, and the resultant structures were refined to 1.7 A resolution. The data demonstrate the feasibility of RT raster-scanning serial micro- and nano-protein crystallography at synchrotrons and validate it as an alternative approach for the collection of high-resolution structural data from micro-sized crystals. Advantages of the proposed approach are its thriftiness, its handling-free nature, the reduced amount of sample required, the adjustable hit rate, the high indexing rate and the minimization of background scattering.
-Authors: Coquelle, N., Kappe, U., Shilova, A., Weinhausen, B., Burghammer, M., Colletier, J.P.
+Raster-scanning serial protein crystallography using micro- and nano-focused synchrotron beams.,Coquelle N, Brewster AS, Kapp U, Shilova A, Weinhausen B, Burghammer M, Colletier JP Acta Crystallogr D Biol Crystallogr. 2015 May;71(Pt 5):1184-96. doi:, 10.1107/S1399004715004514. Epub 2015 Apr 25. PMID:25945583<ref>PMID:25945583</ref>
-Description: Room-temperature crystal structure of lysozyme determined by serial synchrotron crystallography using a nano focused beam.
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Coquelle, N]]
+<div class="pdbe-citations 4wg7" style="background-color:#fffaf0;"></div>
-[[Category: Burghammer, M]]
-[[Category: Shilova, A]]
+==See Also==
-[[Category: Kappe, U]]
+*[[Lysozyme 3D structures|Lysozyme 3D structures]]
-[[Category: Colletier, J.P]]
+== References ==
-[[Category: Weinhausen, B]]
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Gallus gallus]]
+[[Category: Large Structures]]
+[[Category: Brewster AS]]
+[[Category: Burghammer M]]
+[[Category: Colletier JP]]
+[[Category: Coquelle N]]
+[[Category: Kappe U]]
+[[Category: Shilova A]]
+[[Category: Weinhausen B]]