4v5t: Difference between revisions
No edit summary |
No edit summary |
||
| (3 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==X-ray structure of the Grapevine Fanleaf virus== | ==X-ray structure of the Grapevine Fanleaf virus== | ||
<StructureSection load='4v5t' size='340' side='right' caption='[[4v5t]], [[Resolution|resolution]] 3.00Å' scene=''> | <StructureSection load='4v5t' size='340' side='right'caption='[[4v5t]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4v5t]] is a 60 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4v5t]] is a 60 chain structure with sequence from [https://en.wikipedia.org/wiki/Grapevine_fanleaf_virus Grapevine fanleaf virus]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2y7t 2y7t], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2y7u 2y7u] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2y7v 2y7v]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V5T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V5T FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v5t OCA], [https://pdbe.org/4v5t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v5t RCSB], [https://www.ebi.ac.uk/pdbsum/4v5t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v5t ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/POL2_GFLV POL2_GFLV] Protein 2A: implicated in RNA2 replication. Could also be required for nematode transmission of the virus. Movement protein: transports viral genome to neighboring plant cells directly through plasmosdesmata, without any budding. The movement protein allows efficient cell to cell propagation, by bypassing the host cell wall barrier. Acts by forming a tubular structure at the host plasmodesmata, enlarging it enough to allow free passage of virion capsids. | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 17: | Line 17: | ||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4v5t" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
| Line 22: | Line 26: | ||
</StructureSection> | </StructureSection> | ||
[[Category: Grapevine fanleaf virus]] | [[Category: Grapevine fanleaf virus]] | ||
[[Category: Bergdoll | [[Category: Large Structures]] | ||
[[Category: Bron | [[Category: Bergdoll M]] | ||
[[Category: Demangeat | [[Category: Bron P]] | ||
[[Category: Lemaire | [[Category: Demangeat G]] | ||
[[Category: Lorber | [[Category: Lemaire O]] | ||
[[Category: Marmonier | [[Category: Lorber B]] | ||
[[Category: Ritzenthaler | [[Category: Marmonier A]] | ||
[[Category: Sauter | [[Category: Ritzenthaler C]] | ||
[[Category: Schellenberger | [[Category: Sauter C]] | ||
[[Category: Schmitt-Keichinger | [[Category: Schellenberger P]] | ||
[[Category: Trapani | [[Category: Schmitt-Keichinger C]] | ||
[[Category: Trapani S]] | |||
Latest revision as of 13:40, 10 January 2024
X-ray structure of the Grapevine Fanleaf virusX-ray structure of the Grapevine Fanleaf virus
Structural highlights
FunctionPOL2_GFLV Protein 2A: implicated in RNA2 replication. Could also be required for nematode transmission of the virus. Movement protein: transports viral genome to neighboring plant cells directly through plasmosdesmata, without any budding. The movement protein allows efficient cell to cell propagation, by bypassing the host cell wall barrier. Acts by forming a tubular structure at the host plasmodesmata, enlarging it enough to allow free passage of virion capsids. Publication Abstract from PubMedMany animal and plant viruses rely on vectors for their transmission from host to host. Grapevine fanleaf virus (GFLV), a picorna-like virus from plants, is transmitted specifically by the ectoparasitic nematode Xiphinema index. The icosahedral capsid of GFLV, which consists of 60 identical coat protein subunits (CP), carries the determinants of this specificity. Here, we provide novel insight into GFLV transmission by nematodes through a comparative structural and functional analysis of two GFLV variants. We isolated a mutant GFLV strain (GFLV-TD) poorly transmissible by nematodes, and showed that the transmission defect is due to a glycine to aspartate mutation at position 297 (Gly297Asp) in the CP. We next determined the crystal structures of the wild-type GFLV strain F13 at 3.0 A and of GFLV-TD at 2.7 A resolution. The Gly297Asp mutation mapped to an exposed loop at the outer surface of the capsid and did not affect the conformation of the assembled capsid, nor of individual CP molecules. The loop is part of a positively charged pocket that includes a previously identified determinant of transmission. We propose that this pocket is a ligand-binding site with essential function in GFLV transmission by X. index. Our data suggest that perturbation of the electrostatic landscape of this pocket affects the interaction of the virion with specific receptors of the nematode's feeding apparatus, and thereby severely diminishes its transmission efficiency. These data provide a first structural insight into the interactions between a plant virus and a nematode vector. Structural Insights into Viral Determinants of Nematode Mediated Grapevine fanleaf virus Transmission.,Schellenberger P, Sauter C, Lorber B, Bron P, Trapani S, Bergdoll M, Marmonier A, Schmitt-Keichinger C, Lemaire O, Demangeat G, Ritzenthaler C PLoS Pathog. 2011 May;7(5):e1002034. Epub 2011 May 19. PMID:21625570[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||