4uj0: Difference between revisions
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<StructureSection load='4uj0' size='340' side='right'caption='[[4uj0]], [[Resolution|resolution]] 1.70Å' scene=''> | <StructureSection load='4uj0' size='340' side='right'caption='[[4uj0]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4uj0]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4uj0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_lycopersicum Solanum lycopersicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UJ0 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uj0 OCA], [https://pdbe.org/4uj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uj0 RCSB], [https://www.ebi.ac.uk/pdbsum/4uj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uj0 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q40128_SOLLC Q40128_SOLLC] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Solanum lycopersicum]] | ||
[[Category: Hulett | [[Category: Hulett MD]] | ||
[[Category: Kvansakul | [[Category: Kvansakul M]] | ||
[[Category: Lay | [[Category: Lay FT]] | ||
[[Category: Richter | [[Category: Richter V]] | ||
Latest revision as of 13:30, 10 January 2024
Crystal structure of the tomato defensin TPP3Crystal structure of the tomato defensin TPP3
Structural highlights
FunctionPublication Abstract from PubMedDefensins are a class of ubiquitously expressed cationic antimicrobial peptides (CAPs) that play an important role in innate defense. Plant defensins are active against a broad range of microbial pathogens and act via multiple mechanisms, including cell membrane permeabilization. The cytolytic activity of defensins has been proposed to involve interaction with specific lipid components in the target cell wall or membrane and defensin oligomerization. Indeed, the defensin Nicotiana alata defensin 1 (NaD1) binds to a broad range of membrane phosphatidylinositol phosphates and forms an oligomeric complex with phosphatidylinositol (4,5)-bisphosphate (PIP2) that facilitates membrane lysis of both mammalian tumor and fungal cells. Here, we report that the tomato defensin TPP3 has a unique lipid binding profile that is specific for PIP2 with which it forms an oligomeric complex that is critical for cytolytic activity. Structural characterization of TPP3 by X-ray crystallography and site-directed mutagenesis demonstrated that it forms a dimer in a "cationic grip" conformation that specifically accommodates the head group of PIP2 to mediate cooperative higher-order oligomerization and subsequent membrane permeabilization. These findings suggest that certain plant defensins are innate immune receptors for phospholipids and adopt conserved dimeric configurations to mediate PIP2 binding and membrane permeabilization. This mechanism of innate defense may be conserved across defensins from different species. The Tomato Defensin TPP3 Binds Phosphatidylinositol (4,5)-Bisphosphate via a Conserved Dimeric Cationic Grip Conformation To Mediate Cell Lysis.,Baxter AA, Richter V, Lay FT, Poon IK, Adda CG, Veneer PK, Phan TK, Bleackley MR, Anderson MA, Kvansakul M, Hulett MD Mol Cell Biol. 2015 Jun 1;35(11):1964-78. doi: 10.1128/MCB.00282-15. Epub 2015, Mar 23. PMID:25802281[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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