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==Acoustic injectors for drop-on-demand serial femtosecond crystallography==
==Acoustic injectors for drop-on-demand serial femtosecond crystallography==
<StructureSection load='5hqd' size='340' side='right' caption='[[5hqd]], [[Resolution|resolution]] 2.52&Aring;' scene=''>
<StructureSection load='5hqd' size='340' side='right'caption='[[5hqd]], [[Resolution|resolution]] 2.52&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5hqd]] is a 1 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5f80 5f80]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HQD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HQD FirstGlance]. <br>
<table><tr><td colspan='2'>[[5hqd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5f80 5f80]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HQD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HQD FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.52&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hqd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hqd OCA], [http://pdbe.org/5hqd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hqd RCSB], [http://www.ebi.ac.uk/pdbsum/5hqd PDBsum]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hqd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hqd OCA], [https://pdbe.org/5hqd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hqd RCSB], [https://www.ebi.ac.uk/pdbsum/5hqd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hqd ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/V5IRV7_THETH V5IRV7_THETH]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
X-ray free-electron lasers (XFELs) provide very intense X-ray pulses suitable for macromolecular crystallography. Each X-ray pulse typically lasts for tens of femtoseconds and the interval between pulses is many orders of magnitude longer. Here we describe two novel acoustic injection systems that use focused sound waves to eject picoliter to nanoliter crystal-containing droplets out of microplates and into the X-ray pulse from which diffraction data are collected. The on-demand droplet delivery is synchronized to the XFEL pulse scheme, resulting in X-ray pulses intersecting up to 88% of the droplets. We tested several types of samples in a range of crystallization conditions, wherein the overall crystal hit ratio (e.g., fraction of images with observable diffraction patterns) is a function of the microcrystal slurry concentration. We report crystal structures from lysozyme, thermolysin, and stachydrine demethylase (Stc2). Additional samples were screened to demonstrate that these methods can be applied to rare samples.
Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography.,Roessler CG, Agarwal R, Allaire M, Alonso-Mori R, Andi B, Bachega JF, Bommer M, Brewster AS, Browne MC, Chatterjee R, Cho E, Cohen AE, Cowan M, Datwani S, Davidson VL, Defever J, Eaton B, Ellson R, Feng Y, Ghislain LP, Glownia JM, Han G, Hattne J, Hellmich J, Heroux A, Ibrahim M, Kern J, Kuczewski A, Lemke HT, Liu P, Majlof L, McClintock WM, Myers S, Nelsen S, Olechno J, Orville AM, Sauter NK, Soares AS, Soltis SM, Song H, Stearns RG, Tran R, Tsai Y, Uervirojnangkoorn M, Wilmot CM, Yachandra V, Yano J, Yukl ET, Zhu D, Zouni A Structure. 2016 Apr 5;24(4):631-40. doi: 10.1016/j.str.2016.02.007. Epub 2016 Mar, 17. PMID:26996959<ref>PMID:26996959</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5hqd" style="background-color:#fffaf0;"></div>
==See Also==
*[[Thermolysin 3D structures|Thermolysin 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Agarwal, R]]
[[Category: Large Structures]]
[[Category: Allaire, M]]
[[Category: Thermus thermophilus]]
[[Category: Alonso-Mori, R]]
[[Category: Agarwal R]]
[[Category: Andi, B]]
[[Category: Allaire M]]
[[Category: Bachega, J F.R]]
[[Category: Alonso-Mori R]]
[[Category: Bommer, M]]
[[Category: Andi B]]
[[Category: Brewster, A S]]
[[Category: Bachega JFR]]
[[Category: Browne, M C]]
[[Category: Bommer M]]
[[Category: Chatterjee, R]]
[[Category: Brewster AS]]
[[Category: Cho, E]]
[[Category: Browne MC]]
[[Category: Cohen, A E]]
[[Category: Chatterjee R]]
[[Category: Cowan, M]]
[[Category: Cho E]]
[[Category: Datwani, S]]
[[Category: Cohen AE]]
[[Category: Davidson, V L]]
[[Category: Cowan M]]
[[Category: Defever, J]]
[[Category: Datwani S]]
[[Category: Eaton, B]]
[[Category: Davidson VL]]
[[Category: Ellson, R]]
[[Category: Defever J]]
[[Category: Feng, Y]]
[[Category: Eaton B]]
[[Category: Ghislain, L P]]
[[Category: Ellson R]]
[[Category: Glownia, J M]]
[[Category: Feng Y]]
[[Category: Han, G]]
[[Category: Ghislain LP]]
[[Category: Hattne, J]]
[[Category: Glownia JM]]
[[Category: Hellmich, J]]
[[Category: Han G]]
[[Category: Heroux, A]]
[[Category: Hattne J]]
[[Category: Ibrahim, M]]
[[Category: Hellmich J]]
[[Category: Kern, J]]
[[Category: Heroux A]]
[[Category: Kuczewski, A]]
[[Category: Ibrahim M]]
[[Category: Lemke, H T]]
[[Category: Kern J]]
[[Category: Liu, P]]
[[Category: Kuczewski A]]
[[Category: Majlof, L]]
[[Category: Lemke HT]]
[[Category: McClintock, W M]]
[[Category: Liu P]]
[[Category: Myers, S]]
[[Category: Majlof L]]
[[Category: Nelsen, S]]
[[Category: McClintock WM]]
[[Category: Olechno, J]]
[[Category: Myers S]]
[[Category: Orville, A M]]
[[Category: Nelsen S]]
[[Category: Roesser, C G]]
[[Category: Olechno J]]
[[Category: Sauter, N K]]
[[Category: Orville AM]]
[[Category: Soares, A S]]
[[Category: Roesser CG]]
[[Category: Soltis, M S]]
[[Category: Sauter NK]]
[[Category: Song, H]]
[[Category: Soares AS]]
[[Category: Stearns, R G]]
[[Category: Soltis MS]]
[[Category: Tran, R]]
[[Category: Song H]]
[[Category: Tsai, Y]]
[[Category: Stearns RG]]
[[Category: Uervirojnangkoorn, M]]
[[Category: Tran R]]
[[Category: Wilmot, C M]]
[[Category: Tsai Y]]
[[Category: Yachandra, V]]
[[Category: Uervirojnangkoorn M]]
[[Category: Yano, J]]
[[Category: Wilmot CM]]
[[Category: Yukl, E T]]
[[Category: Yachandra V]]
[[Category: Zhu, D]]
[[Category: Yano J]]
[[Category: Zouni, A]]
[[Category: Yukl ET]]
[[Category: Hydrolase]]
[[Category: Zhu D]]
[[Category: Metalloproteinase]]
[[Category: Zouni A]]
[[Category: Xfel]]

Latest revision as of 14:21, 3 January 2024

Acoustic injectors for drop-on-demand serial femtosecond crystallographyAcoustic injectors for drop-on-demand serial femtosecond crystallography

Structural highlights

5hqd is a 1 chain structure with sequence from Thermus thermophilus. This structure supersedes the now removed PDB entry 5f80. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.52Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

V5IRV7_THETH

Publication Abstract from PubMed

X-ray free-electron lasers (XFELs) provide very intense X-ray pulses suitable for macromolecular crystallography. Each X-ray pulse typically lasts for tens of femtoseconds and the interval between pulses is many orders of magnitude longer. Here we describe two novel acoustic injection systems that use focused sound waves to eject picoliter to nanoliter crystal-containing droplets out of microplates and into the X-ray pulse from which diffraction data are collected. The on-demand droplet delivery is synchronized to the XFEL pulse scheme, resulting in X-ray pulses intersecting up to 88% of the droplets. We tested several types of samples in a range of crystallization conditions, wherein the overall crystal hit ratio (e.g., fraction of images with observable diffraction patterns) is a function of the microcrystal slurry concentration. We report crystal structures from lysozyme, thermolysin, and stachydrine demethylase (Stc2). Additional samples were screened to demonstrate that these methods can be applied to rare samples.

Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography.,Roessler CG, Agarwal R, Allaire M, Alonso-Mori R, Andi B, Bachega JF, Bommer M, Brewster AS, Browne MC, Chatterjee R, Cho E, Cohen AE, Cowan M, Datwani S, Davidson VL, Defever J, Eaton B, Ellson R, Feng Y, Ghislain LP, Glownia JM, Han G, Hattne J, Hellmich J, Heroux A, Ibrahim M, Kern J, Kuczewski A, Lemke HT, Liu P, Majlof L, McClintock WM, Myers S, Nelsen S, Olechno J, Orville AM, Sauter NK, Soares AS, Soltis SM, Song H, Stearns RG, Tran R, Tsai Y, Uervirojnangkoorn M, Wilmot CM, Yachandra V, Yano J, Yukl ET, Zhu D, Zouni A Structure. 2016 Apr 5;24(4):631-40. doi: 10.1016/j.str.2016.02.007. Epub 2016 Mar, 17. PMID:26996959[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Roessler CG, Agarwal R, Allaire M, Alonso-Mori R, Andi B, Bachega JF, Bommer M, Brewster AS, Browne MC, Chatterjee R, Cho E, Cohen AE, Cowan M, Datwani S, Davidson VL, Defever J, Eaton B, Ellson R, Feng Y, Ghislain LP, Glownia JM, Han G, Hattne J, Hellmich J, Heroux A, Ibrahim M, Kern J, Kuczewski A, Lemke HT, Liu P, Majlof L, McClintock WM, Myers S, Nelsen S, Olechno J, Orville AM, Sauter NK, Soares AS, Soltis SM, Song H, Stearns RG, Tran R, Tsai Y, Uervirojnangkoorn M, Wilmot CM, Yachandra V, Yano J, Yukl ET, Zhu D, Zouni A. Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography. Structure. 2016 Apr 5;24(4):631-40. doi: 10.1016/j.str.2016.02.007. Epub 2016 Mar, 17. PMID:26996959 doi:http://dx.doi.org/10.1016/j.str.2016.02.007

5hqd, resolution 2.52Å

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