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==Acoustic injectors for drop-on-demand serial femtosecond crystallography==
==Acoustic injectors for drop-on-demand serial femtosecond crystallography==
<StructureSection load='5f81' size='340' side='right' caption='[[5f81]], [[Resolution|resolution]] 2.13&Aring;' scene=''>
<StructureSection load='5f81' size='340' side='right'caption='[[5f81]], [[Resolution|resolution]] 2.13&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5f81]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F81 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F81 FirstGlance]. <br>
<table><tr><td colspan='2'>[[5f81]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5F81 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.13&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5f80|5f80]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5f81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f81 OCA], [https://pdbe.org/5f81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5f81 RCSB], [https://www.ebi.ac.uk/pdbsum/5f81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5f81 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f81 OCA], [http://pdbe.org/5f81 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f81 RCSB], [http://www.ebi.ac.uk/pdbsum/5f81 PDBsum]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>  
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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</div>
</div>
<div class="pdbe-citations 5f81" style="background-color:#fffaf0;"></div>
<div class="pdbe-citations 5f81" style="background-color:#fffaf0;"></div>
==See Also==
*[[Lysozyme 3D structures|Lysozyme 3D structures]]
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
[[Category: Lysozyme]]
[[Category: Large Structures]]
[[Category: Agarwal, R]]
[[Category: Agarwal R]]
[[Category: Allaire, M]]
[[Category: Allaire M]]
[[Category: Alonso-Mori, R]]
[[Category: Alonso-Mori R]]
[[Category: Andi, B]]
[[Category: Andi B]]
[[Category: Bachega, J F.R]]
[[Category: Bachega JFR]]
[[Category: Bommer, M]]
[[Category: Bommer M]]
[[Category: Brewster, A S]]
[[Category: Brewster AS]]
[[Category: Browne, M C]]
[[Category: Browne MC]]
[[Category: Chatterjee, R]]
[[Category: Chatterjee R]]
[[Category: Cho, E]]
[[Category: Cho E]]
[[Category: Cohen, A E]]
[[Category: Cohen AE]]
[[Category: Cowan, M]]
[[Category: Cowan M]]
[[Category: Datwani, S]]
[[Category: Datwani S]]
[[Category: Davidson, V L]]
[[Category: Davidson VL]]
[[Category: Defever, J]]
[[Category: Defever J]]
[[Category: Eaton, B]]
[[Category: Eaton B]]
[[Category: Ellson, R]]
[[Category: Ellson R]]
[[Category: Feng, Y]]
[[Category: Feng Y]]
[[Category: Ghislain, L P]]
[[Category: Ghislain LP]]
[[Category: Glownia, J M]]
[[Category: Glownia JM]]
[[Category: Han, G]]
[[Category: Han G]]
[[Category: Hattne, J]]
[[Category: Hattne J]]
[[Category: Hellmich, J]]
[[Category: Hellmich J]]
[[Category: Heroux, A]]
[[Category: Heroux A]]
[[Category: Ibrahim, M]]
[[Category: Ibrahim M]]
[[Category: Kern, J]]
[[Category: Kern J]]
[[Category: Kuczewski, A]]
[[Category: Kuczewski A]]
[[Category: Lemke, H T]]
[[Category: Lemke HT]]
[[Category: Liu, P]]
[[Category: Liu P]]
[[Category: Majlof, L]]
[[Category: Majlof L]]
[[Category: McClintock, W M]]
[[Category: McClintock WM]]
[[Category: Myers, S]]
[[Category: Myers S]]
[[Category: Nelsen, S]]
[[Category: Nelsen S]]
[[Category: Olechno, J]]
[[Category: Olechno J]]
[[Category: Orville, A M]]
[[Category: Orville AM]]
[[Category: Roessler, C G]]
[[Category: Roessler CG]]
[[Category: Sauter, N K]]
[[Category: Sauter NK]]
[[Category: Soares, A S]]
[[Category: Soares AS]]
[[Category: Soltis, M S]]
[[Category: Soltis MS]]
[[Category: Song, H]]
[[Category: Song H]]
[[Category: Stearns, R G]]
[[Category: Stearns RG]]
[[Category: Tran, R]]
[[Category: Tran R]]
[[Category: Tsai, Y]]
[[Category: Tsai Y]]
[[Category: Uervirojnangkoorn, M]]
[[Category: Uervirojnangkoorn M]]
[[Category: Wilmot, C M]]
[[Category: Wilmot CM]]
[[Category: Yachandra, V]]
[[Category: Yachandra V]]
[[Category: Yano, J]]
[[Category: Yano J]]
[[Category: Yukl, E T]]
[[Category: Yukl ET]]
[[Category: Zhu, D]]
[[Category: Zhu D]]
[[Category: Zouni, A]]
[[Category: Zouni A]]
[[Category: Hydrolase]]

Latest revision as of 14:21, 3 January 2024

Acoustic injectors for drop-on-demand serial femtosecond crystallographyAcoustic injectors for drop-on-demand serial femtosecond crystallography

Structural highlights

5f81 is a 1 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.13Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LYSC_CHICK Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.[1]

Publication Abstract from PubMed

X-ray free-electron lasers (XFELs) provide very intense X-ray pulses suitable for macromolecular crystallography. Each X-ray pulse typically lasts for tens of femtoseconds and the interval between pulses is many orders of magnitude longer. Here we describe two novel acoustic injection systems that use focused sound waves to eject picoliter to nanoliter crystal-containing droplets out of microplates and into the X-ray pulse from which diffraction data are collected. The on-demand droplet delivery is synchronized to the XFEL pulse scheme, resulting in X-ray pulses intersecting up to 88% of the droplets. We tested several types of samples in a range of crystallization conditions, wherein the overall crystal hit ratio (e.g., fraction of images with observable diffraction patterns) is a function of the microcrystal slurry concentration. We report crystal structures from lysozyme, thermolysin, and stachydrine demethylase (Stc2). Additional samples were screened to demonstrate that these methods can be applied to rare samples.

Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography.,Roessler CG, Agarwal R, Allaire M, Alonso-Mori R, Andi B, Bachega JF, Bommer M, Brewster AS, Browne MC, Chatterjee R, Cho E, Cohen AE, Cowan M, Datwani S, Davidson VL, Defever J, Eaton B, Ellson R, Feng Y, Ghislain LP, Glownia JM, Han G, Hattne J, Hellmich J, Heroux A, Ibrahim M, Kern J, Kuczewski A, Lemke HT, Liu P, Majlof L, McClintock WM, Myers S, Nelsen S, Olechno J, Orville AM, Sauter NK, Soares AS, Soltis SM, Song H, Stearns RG, Tran R, Tsai Y, Uervirojnangkoorn M, Wilmot CM, Yachandra V, Yano J, Yukl ET, Zhu D, Zouni A Structure. 2016 Apr 5;24(4):631-40. doi: 10.1016/j.str.2016.02.007. Epub 2016 Mar, 17. PMID:26996959[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Maehashi K, Matano M, Irisawa T, Uchino M, Kashiwagi Y, Watanabe T. Molecular characterization of goose- and chicken-type lysozymes in emu (Dromaius novaehollandiae): evidence for extremely low lysozyme levels in emu egg white. Gene. 2012 Jan 15;492(1):244-9. doi: 10.1016/j.gene.2011.10.021. Epub 2011 Oct, 25. PMID:22044478 doi:10.1016/j.gene.2011.10.021
  2. ↑ Roessler CG, Agarwal R, Allaire M, Alonso-Mori R, Andi B, Bachega JF, Bommer M, Brewster AS, Browne MC, Chatterjee R, Cho E, Cohen AE, Cowan M, Datwani S, Davidson VL, Defever J, Eaton B, Ellson R, Feng Y, Ghislain LP, Glownia JM, Han G, Hattne J, Hellmich J, Heroux A, Ibrahim M, Kern J, Kuczewski A, Lemke HT, Liu P, Majlof L, McClintock WM, Myers S, Nelsen S, Olechno J, Orville AM, Sauter NK, Soares AS, Soltis SM, Song H, Stearns RG, Tran R, Tsai Y, Uervirojnangkoorn M, Wilmot CM, Yachandra V, Yano J, Yukl ET, Zhu D, Zouni A. Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography. Structure. 2016 Apr 5;24(4):631-40. doi: 10.1016/j.str.2016.02.007. Epub 2016 Mar, 17. PMID:26996959 doi:http://dx.doi.org/10.1016/j.str.2016.02.007

5f81, resolution 2.13Å

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