4wnb: Difference between revisions
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==Crystal structure of the ChsH1-ChsH2 complex from Mycobacterium tuberculosis bound to 3-OPC-CoA== | ==Crystal structure of the ChsH1-ChsH2 complex from Mycobacterium tuberculosis bound to 3-OPC-CoA== | ||
<StructureSection load='4wnb' size='340' side='right' caption='[[4wnb]], [[Resolution|resolution]] 1.76Å' scene=''> | <StructureSection load='4wnb' size='340' side='right'caption='[[4wnb]], [[Resolution|resolution]] 1.76Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4wnb]] is a 2 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4w7b 4w7b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WNB OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[4wnb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_C Mycobacterium tuberculosis C] and [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_KZN_4207 Mycobacterium tuberculosis KZN 4207]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4w7b 4w7b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WNB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WNB FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4BN:3-OXO-4-PREGNENE-20-CARBOXYL-COENZYME+A'>4BN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4BN:3-OXO-4-PREGNENE-20-CARBOXYL-COENZYME+A'>4BN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wnb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wnb OCA], [https://pdbe.org/4wnb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wnb RCSB], [https://www.ebi.ac.uk/pdbsum/4wnb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wnb ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
| Line 20: | Line 21: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Garcia-Diaz | [[Category: Large Structures]] | ||
[[Category: Guja | [[Category: Mycobacterium tuberculosis C]] | ||
[[Category: Sampson | [[Category: Mycobacterium tuberculosis KZN 4207]] | ||
[[Category: Yang | [[Category: Garcia-Diaz M]] | ||
[[Category: Guja KE]] | |||
[[Category: Sampson N]] | |||
[[Category: Yang M]] | |||
Latest revision as of 03:54, 28 December 2023
Crystal structure of the ChsH1-ChsH2 complex from Mycobacterium tuberculosis bound to 3-OPC-CoACrystal structure of the ChsH1-ChsH2 complex from Mycobacterium tuberculosis bound to 3-OPC-CoA
Structural highlights
Publication Abstract from PubMedThe Mycobacterium tuberculosis (Mtb) igr operon plays an essential role in Mtb cholesterol metabolism, which is critical for pathogenesis during the latent stage of Mtb infection. Here we report the first structure of a heterotetrameric MaoC-like enoyl-CoA hydratase, ChsH1-ChsH2, which is encoded by two adjacent genes from the igr operon. We demonstrate that ChsH1-ChsH2 catalyzes the hydration of a steroid enoyl-CoA, 3-oxo-4,17-pregnadiene-20-carboxyl-CoA, in the modified beta-oxidation pathway for cholesterol side chain degradation. The ligand-bound and apoenzyme structures of ChsH1-ChsH2N reveal an unusual, modified hot-dog fold with a severely truncated central alpha-helix that creates an expanded binding site to accommodate the bulkier steroid ring system. The structures show quaternary structure shifts that accommodate the four rings of the steroid substrate and offer an explanation for why the unusual heterotetrameric assembly is utilized for hydration of this steroid. The unique alphabeta heterodimer architecture utilized by ChsH1-ChsH2 to bind its distinctive substrate highlights an opportunity for the development of new antimycobacterial drugs that target a pathway specific to Mtb. A Distinct MaoC-like Enoyl-CoA Hydratase Architecture Mediates Cholesterol Catabolism in Mycobacterium tuberculosis.,Yang M, Guja KE, Thomas ST, Garcia-Diaz M, Sampson NS ACS Chem Biol. 2014 Sep 22. PMID:25203216[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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