4plx: Difference between revisions
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==Crystal structure of the triple-helical stability element at the 3' end of MALAT1== | |||
<StructureSection load='4plx' size='340' side='right'caption='[[4plx]], [[Resolution|resolution]] 3.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4plx]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PLX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PLX FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A23:ADENOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>A23</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4plx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4plx OCA], [https://pdbe.org/4plx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4plx RCSB], [https://www.ebi.ac.uk/pdbsum/4plx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4plx ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a highly abundant nuclear long noncoding RNA that promotes malignancy. A 3'-stem-loop structure is predicted to confer stability by engaging a downstream A-rich tract in a triple helix, similar to the expression and nuclear retention element (ENE) from the KSHV polyadenylated nuclear RNA. The 3.1-A-resolution crystal structure of the human MALAT1 ENE and A-rich tract reveals a bipartite triple helix containing stacks of five and four U*A-U triples separated by a C(+)*G-C triplet and C-G doublet, extended by two A-minor interactions. In vivo decay assays indicate that this blunt-ended triple helix, with the 3' nucleotide in a U*A-U triple, inhibits rapid nuclear RNA decay. Interruption of the triple helix by the C-G doublet induces a 'helical reset' that explains why triple-helical stacks longer than six do not occur in nature. | |||
Structural insights into the stabilization of MALAT1 noncoding RNA by a bipartite triple helix.,Brown JA, Bulkley D, Wang J, Valenstein ML, Yario TA, Steitz TA, Steitz JA Nat Struct Mol Biol. 2014 Jul;21(7):633-40. doi: 10.1038/nsmb.2844. Epub 2014 Jun, 22. PMID:24952594<ref>PMID:24952594</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4plx" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Brown JA]] | |||
[[Category: Bulkley D]] | |||
[[Category: Steitz JA]] | |||
[[Category: Steitz TA]] | |||
[[Category: Valenstein ML]] | |||
[[Category: Wang J]] | |||
[[Category: Yario TA]] | |||
Latest revision as of 03:45, 28 December 2023
Crystal structure of the triple-helical stability element at the 3' end of MALAT1Crystal structure of the triple-helical stability element at the 3' end of MALAT1
Structural highlights
Publication Abstract from PubMedMetastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a highly abundant nuclear long noncoding RNA that promotes malignancy. A 3'-stem-loop structure is predicted to confer stability by engaging a downstream A-rich tract in a triple helix, similar to the expression and nuclear retention element (ENE) from the KSHV polyadenylated nuclear RNA. The 3.1-A-resolution crystal structure of the human MALAT1 ENE and A-rich tract reveals a bipartite triple helix containing stacks of five and four U*A-U triples separated by a C(+)*G-C triplet and C-G doublet, extended by two A-minor interactions. In vivo decay assays indicate that this blunt-ended triple helix, with the 3' nucleotide in a U*A-U triple, inhibits rapid nuclear RNA decay. Interruption of the triple helix by the C-G doublet induces a 'helical reset' that explains why triple-helical stacks longer than six do not occur in nature. Structural insights into the stabilization of MALAT1 noncoding RNA by a bipartite triple helix.,Brown JA, Bulkley D, Wang J, Valenstein ML, Yario TA, Steitz TA, Steitz JA Nat Struct Mol Biol. 2014 Jul;21(7):633-40. doi: 10.1038/nsmb.2844. Epub 2014 Jun, 22. PMID:24952594[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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