4pd3: Difference between revisions
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==Crystal Structure of Rigor-Like Human Nonmuscle Myosin-2B== | |||
<StructureSection load='4pd3' size='340' side='right'caption='[[4pd3]], [[Resolution|resolution]] 2.84Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4pd3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PD3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PD3 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.84Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pd3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pd3 OCA], [https://pdbe.org/4pd3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pd3 RCSB], [https://www.ebi.ac.uk/pdbsum/4pd3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pd3 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/ACTNA_DICDI ACTNA_DICDI] F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Increases the actin-stimulated ATPase activity of myosin. Involved in vegetative cell growth, phagocytosis, motility and development, probably through stabilization of the actin network in the cortical cytoskeleton.<ref>PMID:10411959</ref> <ref>PMID:10413681</ref> <ref>PMID:10704840</ref> <ref>PMID:1732064</ref> <ref>PMID:3956480</ref> <ref>PMID:6746725</ref> <ref>PMID:8486739</ref> <ref>PMID:8937986</ref> [https://www.uniprot.org/uniprot/MYH10_HUMAN MYH10_HUMAN] Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the central part but not the margins of spreading cells), and lamellipodial extension; this function is mechanically antagonized by MYH9.<ref>PMID:20052411</ref> <ref>PMID:20603131</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We determined the crystal structure of the motor domain of human non-muscle myosin 2B (NM-2B) in a nucleotide-free state and at a resolution of 2.8A. The structure shows the motor domain with an open active site and the large cleft that divides the 50kDa domain in a closed state. Compared to other rigor-like myosin motor domain structures, our structure shows subtle but significant conformational changes in regions important for actin binding and mechanochemical coupling. Moreover, our crystal structure helps to rationalize the impact of myosin, heavy chain 9 (MYH9)-related disease mutations Arg709Cys and Arg709His on the kinetic and functional properties of NM-2B and of the closely related non-muscle myosin 2A (NM-2A). | |||
Crystal structure of the rigor-like human non-muscle myosin-2 motor domain.,Munnich S, Pathan-Chhatbar S, Manstein DJ FEBS Lett. 2014 Dec 20;588(24):4754-60. doi: 10.1016/j.febslet.2014.11.007. Epub , 2014 Nov 15. PMID:25451231<ref>PMID:25451231</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4pd3" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Actinin 3D structures|Actinin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Dictyostelium discoideum]] | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Manstein DJ]] | |||
[[Category: Munnich S]] | |||
[[Category: Pathan-Chhatbar S]] | |||