3lyn: Difference between revisions
New page: left|200px<br /><applet load="3lyn" size="450" color="white" frame="true" align="right" spinBox="true" caption="3lyn, resolution 1.70Å" /> '''STRUCTURE OF GREEN A... |
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== | ==STRUCTURE OF GREEN ABALONE LYSIN DIMER== | ||
Abalone sperm lysin is a 16 kDa acrosomal protein used by sperm to create | <StructureSection load='3lyn' size='340' side='right'caption='[[3lyn]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3lyn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Haliotis_fulgens Haliotis fulgens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LYN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LYN FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3lyn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lyn OCA], [https://pdbe.org/3lyn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3lyn RCSB], [https://www.ebi.ac.uk/pdbsum/3lyn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3lyn ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/ELYS_HALFU ELYS_HALFU] Dissolves the egg vitelline layer nonenzymatically during fertilization. It creates a hole of about 3 mu-m in diameter through which the sperm pass. | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ly/3lyn_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3lyn ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Abalone sperm lysin is a 16 kDa acrosomal protein used by sperm to create a hole in the egg vitelline envelope. Lysins from seven California abalone exhibit species-specificity in binding to their egg receptor, and range in sequence identity from 63 % to 90 %. The crystal structure of the sperm lysin dimer from Haliotis fulgens (green abalone) has been determined to 1.71 A by multiple isomorphous replacement. Comparisons with the structure of the lysin dimer from Haliotis rufescens (red abalone) reveal a similar overall fold and conservation of features contributing to lysin's amphipathic character. The two structures do, however, exhibit differences in surface residues and electrostatics. A large clustering of non-conserved surface residues around the waist and clefts of the dimer, and differences in charged residues around these regions, indicate areas of the molecule which may be involved in species-specific egg recognition. | |||
The high resolution crystal structure of green abalone sperm lysin: implications for species-specific binding of the egg receptor.,Kresge N, Vacquier VD, Stout CD J Mol Biol. 2000 Mar 10;296(5):1225-34. PMID:10698629<ref>PMID:10698629</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3lyn" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Lysin 3D structures|Lysin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Haliotis fulgens]] | [[Category: Haliotis fulgens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Kresge | [[Category: Kresge N]] | ||
[[Category: Stout | [[Category: Stout CD]] | ||
[[Category: Vacquier | [[Category: Vacquier VD]] | ||
