3ews: Difference between revisions
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== | ==Human DEAD-box RNA-helicase DDX19 in complex with ADP== | ||
[[3ews]] is a 2 chain structure with sequence from [ | <StructureSection load='3ews' size='340' side='right'caption='[[3ews]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3ews]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EWS FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ews FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ews OCA], [https://pdbe.org/3ews PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ews RCSB], [https://www.ebi.ac.uk/pdbsum/3ews PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ews ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/DD19B_HUMAN DD19B_HUMAN] ATP-dependent RNA helicase involved in mRNA export from the nucleus. Rather than unwinding RNA duplexes, DDX19B functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins. | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/3ews_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ews ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
DEXD/H-box RNA helicases couple ATP hydrolysis to RNA remodeling by an unknown mechanism. We used x-ray crystallography and biochemical analysis of the human DEXD/H-box protein DDX19 to investigate its regulatory mechanism. The crystal structures of DDX19, in its RNA-bound prehydrolysis and free posthydrolysis state, reveal an alpha-helix that inserts between the conserved domains of the free protein to negatively regulate ATPase activity. This finding was corroborated by biochemical data that confirm an autoregulatory function of the N-terminal region of the protein. This is the first study describing crystal structures of a DEXD/H-box protein in its open and closed cleft conformations. | |||
The DEXD/H-box RNA helicase DDX19 is regulated by an {alpha}-helical switch.,Collins R, Karlberg T, Lehtio L, Schutz P, van den Berg S, Dahlgren LG, Hammarstrom M, Weigelt J, Schuler H J Biol Chem. 2009 Apr 17;284(16):10296-300. Epub 2009 Feb 25. PMID:19244245<ref>PMID:19244245</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3ews" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
*[[Helicase 3D structures|Helicase 3D structures]] | |||
== References == | |||
*[[Helicase|Helicase]] | <references/> | ||
__TOC__ | |||
== | </StructureSection> | ||
< | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Andersson | [[Category: Large Structures]] | ||
[[Category: Arrowsmith | [[Category: Andersson j]] | ||
[[Category: Berglund | [[Category: Arrowsmith ch]] | ||
[[Category: Bountra | [[Category: Berglund h]] | ||
[[Category: Collins | [[Category: Bountra c]] | ||
[[Category: Dahlgren | [[Category: Collins r]] | ||
[[Category: Edwards | [[Category: Dahlgren lg]] | ||
[[Category: Flodin | [[Category: Edwards am]] | ||
[[Category: Flores | [[Category: Flodin s]] | ||
[[Category: Graslund | [[Category: Flores a]] | ||
[[Category: Hammarstrom | [[Category: Graslund s]] | ||
[[Category: Johansson | [[Category: Hammarstrom m]] | ||
[[Category: Johansson | [[Category: Johansson a]] | ||
[[Category: Karlberg | [[Category: Johansson i]] | ||
[[Category: Kotenyova | [[Category: Karlberg t]] | ||
[[Category: Lehtio | [[Category: Kotenyova t]] | ||
[[Category: Moche | [[Category: Lehtio l]] | ||
[[Category: Nilsson | [[Category: Moche m]] | ||
[[Category: Nordlund | [[Category: Nilsson me]] | ||
[[Category: Nyman | [[Category: Nordlund p]] | ||
[[Category: Olesen | [[Category: Nyman t]] | ||
[[Category: Persson | [[Category: Olesen k]] | ||
[[Category: Persson c]] | |||
[[Category: Sagemark | [[Category: Sagemark j]] | ||
[[Category: Schueler | [[Category: Schueler h]] | ||
[[Category: Thorsell | [[Category: Thorsell ag]] | ||
[[Category: Tresaugues | [[Category: Tresaugues l]] | ||
[[Category: | [[Category: Van den berg s]] | ||
[[Category: | [[Category: Weigelt j]] | ||
[[Category: | [[Category: Welin m]] | ||
[[Category: | [[Category: Wikstrom m]] | ||
[[Category: | [[Category: Wisniewska m]] | ||