1cn7: Difference between revisions

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-[[Image:1cn7.gif|left|200px]]
- {{Structure
+==Yeast ribosomal protein L30==
-|PDB= 1cn7 |SIZE=350|CAPTION= <scene name='initialview01'>1cn7</scene>
+<StructureSection load='1cn7' size='340' side='right'caption='[[1cn7]]' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND=
+<table><tr><td colspan='2'>[[1cn7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ck9 1ck9]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CN7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CN7 FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-|GENE= RPL30 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cn7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cn7 OCA], [https://pdbe.org/1cn7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cn7 RCSB], [https://www.ebi.ac.uk/pdbsum/1cn7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cn7 ProSAT]</span></td></tr>
-|DOMAIN=
+</table>
-|RELATEDENTRY=
+== Function ==
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cn7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cn7 OCA], [http://www.ebi.ac.uk/pdbsum/1cn7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1cn7 RCSB]</span>
+[https://www.uniprot.org/uniprot/RL30_YEAST RL30_YEAST]
-}}
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cn/1cn7_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cn7 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The ribosomal protein L30 from yeast Saccharomyces cerevisiae auto-regulates its own synthesis by binding to a structural element in both its pre-mRNA and its mRNA. The three-dimensional structures of L30 in the free (f L30) and the pre-mRNA bound (b L30) forms have been solved by nuclear magnetic resonance spectroscopy. Both protein structures contain four alternating alpha-helices and four beta-strands segments and adopt an overall topology that is an alphabetaalpha three-layer sandwich, representing a unique fold. Three loops on one end of the alphabetaalpha sandwich have been mapped as the RNA binding site on the basis of structural comparison, chemical shift perturbation and the inter-molecular nuclear Overhauser effects to the RNA. The structural and dynamic comparison of f L30 and b L30 reveals that local dynamics may play an important role in the RNA binding. The fourth helix in b L30 is longer than in f L30, and is stabilized by RNA binding. The exposed hydrophobic surface that is buried upon RNA binding may provide the energy necessary to drive secondary structure formation, and may account for the increased stability of b L30.
-'''Yeast ribosomal protein L30'''
+Local folding coupled to RNA binding in the yeast ribosomal protein L30.,Mao H, Williamson JR J Mol Biol. 1999 Sep 17;292(2):345-59. PMID:10493880<ref>PMID:10493880</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1cn7" style="background-color:#fffaf0;"></div>
-==Overview==
+==See Also==
-The ribosomal protein L30 from yeast Saccharomyces cerevisiae auto-regulates its own synthesis by binding to a structural element in both its pre-mRNA and its mRNA. The three-dimensional structures of L30 in the free (f L30) and the pre-mRNA bound (b L30) forms have been solved by nuclear magnetic resonance spectroscopy. Both protein structures contain four alternating alpha-helices and four beta-strands segments and adopt an overall topology that is an alphabetaalpha three-layer sandwich, representing a unique fold. Three loops on one end of the alphabetaalpha sandwich have been mapped as the RNA binding site on the basis of structural comparison, chemical shift perturbation and the inter-molecular nuclear Overhauser effects to the RNA. The structural and dynamic comparison of f L30 and b L30 reveals that local dynamics may play an important role in the RNA binding. The fourth helix in b L30 is longer than in f L30, and is stabilized by RNA binding. The exposed hydrophobic surface that is buried upon RNA binding may provide the energy necessary to drive secondary structure formation, and may account for the increased stability of b L30.
+*[[Ribosomal protein L30|Ribosomal protein L30]]
+== References ==
-==About this Structure==
+<references/>
-CN7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. This structure supersedes the now removed PDB entry 1CK9. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CN7 OCA].
+__TOC__
+</StructureSection>
-==Reference==
+[[Category: Large Structures]]
-Local folding coupled to RNA binding in the yeast ribosomal protein L30., Mao H, Williamson JR, J Mol Biol. 1999 Sep 17;292(2):345-59. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10493880 10493880]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Single protein]]
+[[Category: Mao H]]
-[[Category: Mao, H.]]
+[[Category: Willamson JR]]
-[[Category: Willamson, J R.]]
-[[Category: auto-regulation of pre-mrna splicing and mrna translation]]
-[[Category: nmr]]
-[[Category: ribosomal protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:24:51 2008''