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[[Image:2qh4.gif|left|200px]]
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{{STRUCTURE_2qh4|  PDB=2qh4  |  SCENE=  }}
'''Solution structure of the U85 C/D-H/ACA scaRNA 5' terminal hairpin loop'''


==Solution structure of the U85 C/D-H/ACA scaRNA 5' terminal hairpin loop==
<StructureSection load='2qh4' size='340' side='right'caption='[[2qh4]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2qh4]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QH4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qh4 OCA], [https://pdbe.org/2qh4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qh4 RCSB], [https://www.ebi.ac.uk/pdbsum/2qh4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qh4 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The RNA component of human telomerase (hTR) includes H/ACA and CR7 domains required for 3' end processing, localization, and accumulation. The terminal loop of the CR7 domain contains the CAB box (ugAG) required for targeting of scaRNAs to Cajal bodies (CB) and an uncharacterized sequence required for accumulation and processing. To dissect out the contributions of the CR7 stem loop to hTR processing and localization, we solved the solution structures of the 3' terminal stem loops of hTR CR7 and U64 H/ACA snoRNA, and the 5' terminal stem loop of U85 C/D-H/ACA scaRNA. These structures, together with analysis of localization, processing, and accumulation of hTRs containing nucleotide substitutions in the CR7 domain, identified the sequence and structural requirements of the hTR processing and CB localization signals and showed that these signals are functionally independent. Further, 3' end processing was found to be a prerequisite for translocation of hTR to CBs.


==Overview==
Structural and functional characterization of human telomerase RNA processing and cajal body localization signals.,Theimer CA, Jady BE, Chim N, Richard P, Breece KE, Kiss T, Feigon J Mol Cell. 2007 Sep 21;27(6):869-81. PMID:17889661<ref>PMID:17889661</ref>
The RNA component of human telomerase (hTR) includes H/ACA and CR7 domains required for 3' end processing, localization, and accumulation. The terminal loop of the CR7 domain contains the CAB box (ugAG) required for targeting of scaRNAs to Cajal bodies (CB) and an uncharacterized sequence required for accumulation and processing. To dissect out the contributions of the CR7 stem loop to hTR processing and localization, we solved the solution structures of the 3' terminal stem loops of hTR CR7 and U64 H/ACA snoRNA, and the 5' terminal stem loop of U85 C/D-H/ACA scaRNA. These structures, together with analysis of localization, processing, and accumulation of hTRs containing nucleotide substitutions in the CR7 domain, identified the sequence and structural requirements of the hTR processing and CB localization signals and showed that these signals are functionally independent. Further, 3' end processing was found to be a prerequisite for translocation of hTR to CBs.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QH4 OCA].
</div>
<div class="pdbe-citations 2qh4" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structural and functional characterization of human telomerase RNA processing and cajal body localization signals., Theimer CA, Jady BE, Chim N, Richard P, Breece KE, Kiss T, Feigon J, Mol Cell. 2007 Sep 21;27(6):869-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17889661 17889661]
*[[Telomerase 3D structures|Telomerase 3D structures]]
[[Category: Breece, K E.]]
== References ==
[[Category: Chim, N.]]
<references/>
[[Category: Feigon, J.]]
__TOC__
[[Category: Theimer, C A.]]
</StructureSection>
[[Category: Loop]]
[[Category: Large Structures]]
[[Category: Rna]]
[[Category: Breece KE]]
[[Category: Sheared ga pair]]
[[Category: Chim N]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 14:57:48 2008''
[[Category: Feigon J]]
[[Category: Theimer CA]]

Latest revision as of 15:53, 20 December 2023

Solution structure of the U85 C/D-H/ACA scaRNA 5' terminal hairpin loopSolution structure of the U85 C/D-H/ACA scaRNA 5' terminal hairpin loop

Structural highlights

2qh4 is a 1 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The RNA component of human telomerase (hTR) includes H/ACA and CR7 domains required for 3' end processing, localization, and accumulation. The terminal loop of the CR7 domain contains the CAB box (ugAG) required for targeting of scaRNAs to Cajal bodies (CB) and an uncharacterized sequence required for accumulation and processing. To dissect out the contributions of the CR7 stem loop to hTR processing and localization, we solved the solution structures of the 3' terminal stem loops of hTR CR7 and U64 H/ACA snoRNA, and the 5' terminal stem loop of U85 C/D-H/ACA scaRNA. These structures, together with analysis of localization, processing, and accumulation of hTRs containing nucleotide substitutions in the CR7 domain, identified the sequence and structural requirements of the hTR processing and CB localization signals and showed that these signals are functionally independent. Further, 3' end processing was found to be a prerequisite for translocation of hTR to CBs.

Structural and functional characterization of human telomerase RNA processing and cajal body localization signals.,Theimer CA, Jady BE, Chim N, Richard P, Breece KE, Kiss T, Feigon J Mol Cell. 2007 Sep 21;27(6):869-81. PMID:17889661[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Theimer CA, Jady BE, Chim N, Richard P, Breece KE, Kiss T, Feigon J. Structural and functional characterization of human telomerase RNA processing and cajal body localization signals. Mol Cell. 2007 Sep 21;27(6):869-81. PMID:17889661 doi:S1097-2765(07)00490-X
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