2ojn: Difference between revisions

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-{{Seed}}
-[[Image:2ojn.png|left|200px]]
-<!--
+==Solution structure and cell selectivity of Piscidin 1 and its analogues==
-The line below this paragraph, containing "STRUCTURE_2ojn", creates the "Structure Box" on the page.
+<StructureSection load='2ojn' size='340' side='right'caption='[[2ojn]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2ojn]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OJN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OJN FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ojn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ojn OCA], [https://pdbe.org/2ojn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ojn RCSB], [https://www.ebi.ac.uk/pdbsum/2ojn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ojn ProSAT]</span></td></tr>
-{{STRUCTURE_2ojn|  PDB=2ojn  |  SCENE=  }}
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Piscidin 1 (Pis-1) is a novel cytotoxic peptide with a cationic alpha-helical structure that was isolated from the mast cells of hybrid striped bass [Silphaduang, U., and Noga, E. J. (2001) Nature 414, 268-269]. Pis-1 is not selective for bacterial versus mammalian cells. In the present study, to develop novel antibiotic peptides with selectivity for bacterial cells, we examined the effect of substituting two glycine residues, Gly8 and Gly13, with Ala or Pro on this peptide's structure and biological activities. The bacterial cell selectivity of the peptides decreased in the following order: Gly--&gt;Pro analogues &gt; Gly--&gt;Pro/Ala analogues &gt; Pis-1 &gt; Gly--&gt;Ala analogues. The antimicrobial and hemolytic activities and abilities to permeabilize the model phospholipid membranes were higher for Pis-1 with Gly or Pro at position 8 than for its counterparts with either Gly or Pro at position 13. We determined the tertiary structure of Pis-1 and its analogues in the presence of SDS micelles by NMR spectroscopy. We found that Pis-1 has an alpha-helical structure from Phe2 to Thr21. Also, Pis-1 AA (Gly8, Gly13--&gt;Ala8, Ala13) with higher antibacterial and hemolytic activity than Pis-1 has a stable alpha-helical structure from Phe2 to Thr21. Pis-1 PG (Gly--&gt;Pro8) with bacterial cell selectivity has a hinge structure at Pro8, which provides flexibility in piscidin, followed by a three-turn helix from Val10 to Gly22 in the C-terminal region. Taken together, our results demonstrate that the conformational flexibility provided by introduction of a Pro at position 8, coupled with the primary anchoring of phenylalanines and histidines in the N-terminus to the cell membrane and the optimal length of the C-terminal amphipathic alpha-helix, are the critical factors that confer antibacterial activity and bacterial cell selectivity to Pis-1 PG. Pis-1 PG may be a good candidate for the development of a new drug with potent antibacterial activity but without cytotoxicity.
-===Solution structure and cell selectivity of Piscidin 1 and its analogues===
+Solution structure and cell selectivity of piscidin 1 and its analogues.,Lee SA, Kim YK, Lim SS, Zhu WL, Ko H, Shin SY, Hahm KS, Kim Y Biochemistry. 2007 Mar 27;46(12):3653-63. Epub 2007 Mar 1. PMID:17328560<ref>PMID:17328560</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_17328560}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 2ojn" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 17328560 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_17328560}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OJN OCA].
+[[Category: Kim Y]]
+[[Category: Kim YK]]
-==Reference==
+[[Category: Lee SA]]
-Solution structure and cell selectivity of piscidin 1 and its analogues., Lee SA, Kim YK, Lim SS, Zhu WL, Ko H, Shin SY, Hahm KS, Kim Y, Biochemistry. 2007 Mar 27;46(12):3653-63. Epub 2007 Mar 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17328560 17328560]
-[[Category: Kim, Y.]]
-[[Category: Kim, Y K.]]
-[[Category: Lee, S A.]]
-[[Category: Antimicrobial peptide]]
-[[Category: Antimicrobial protein]]
-[[Category: Membrane]]
-[[Category: Piscidin 1]]
-[[Category: Proline]]
-[[Category: Structure]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:00:39 2008''