2kb7: Difference between revisions
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< | ==Hybrid solution and solid-state NMR structure of monomeric phospholamban in lipid bilayers== | ||
<StructureSection load='2kb7' size='340' side='right'caption='[[2kb7]]' scene=''> | |||
== Structural highlights == | |||
or the | <table><tr><td colspan='2'>[[2kb7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KB7 FirstGlance]. <br> | ||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Hybrid , Solid-state NMR , Solution NMR</td></tr> | |||
-- | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kb7 OCA], [https://pdbe.org/2kb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kb7 RCSB], [https://www.ebi.ac.uk/pdbsum/2kb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kb7 ProSAT]</span></td></tr> | ||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/PPLA_RABIT PPLA_RABIT] Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Phospholamban (PLN) is an essential regulator of cardiac muscle contractility. The homopentameric assembly of PLN is the reservoir for active monomers that, upon deoligomerization form 1:1 complexes with the sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA), thus modulating the rate of calcium uptake. In lipid bilayers and micelles, monomeric PLN exists in equilibrium between a bent (or resting) T state and a more dynamic (or active) R state. Here, we report the high-resolution structure and topology of the T state of a monomeric PLN mutant in lipid bilayers, using a hybrid of solution and solid-state NMR restraints together with molecular dynamics simulations in explicit lipid environments. Unlike the previous structural ensemble determined in micelles, this approach gives a complete picture of the PLN monomer structure in a lipid bilayer. This hybrid ensemble exemplifies the tilt, rotation, and depth of membrane insertion, revealing the interaction with the lipids for all protein domains. The N-terminal amphipathic helical domain Ia (residues 1-16) rests on the surface of the lipid membrane with the hydrophobic face of domain Ia embedded in the membrane bilayer interior. The helix comprised of domain Ib (residues 23-30) and transmembrane domain II (residues 31-52) traverses the bilayer with a tilt angle of approximately 24 degrees . The specific interactions between PLN and lipid membranes may represent an additional regulatory element of its inhibitory function. We propose this hybrid method for the simultaneous determination of structure and topology for membrane proteins with compact folds or proteins whose spatial arrangement is dictated by their specific interactions with lipid bilayers. | |||
Structure and topology of monomeric phospholamban in lipid membranes determined by a hybrid solution and solid-state NMR approach.,Traaseth NJ, Shi L, Verardi R, Mullen DG, Barany G, Veglia G Proc Natl Acad Sci U S A. 2009 Jun 23;106(25):10165-70. Epub 2009 Jun 9. PMID:19509339<ref>PMID:19509339</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2kb7" style="background-color:#fffaf0;"></div> | |||
== | ==See Also== | ||
*[[Phospholamban|Phospholamban]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Shi | [[Category: Large Structures]] | ||
[[Category: Traaseth | [[Category: Shi L]] | ||
[[Category: Veglia | [[Category: Traaseth NJ]] | ||
[[Category: Verardi | [[Category: Veglia G]] | ||
[[Category: Verardi R]] | |||
Latest revision as of 15:50, 20 December 2023
Hybrid solution and solid-state NMR structure of monomeric phospholamban in lipid bilayersHybrid solution and solid-state NMR structure of monomeric phospholamban in lipid bilayers
Structural highlights
FunctionPPLA_RABIT Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. Publication Abstract from PubMedPhospholamban (PLN) is an essential regulator of cardiac muscle contractility. The homopentameric assembly of PLN is the reservoir for active monomers that, upon deoligomerization form 1:1 complexes with the sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA), thus modulating the rate of calcium uptake. In lipid bilayers and micelles, monomeric PLN exists in equilibrium between a bent (or resting) T state and a more dynamic (or active) R state. Here, we report the high-resolution structure and topology of the T state of a monomeric PLN mutant in lipid bilayers, using a hybrid of solution and solid-state NMR restraints together with molecular dynamics simulations in explicit lipid environments. Unlike the previous structural ensemble determined in micelles, this approach gives a complete picture of the PLN monomer structure in a lipid bilayer. This hybrid ensemble exemplifies the tilt, rotation, and depth of membrane insertion, revealing the interaction with the lipids for all protein domains. The N-terminal amphipathic helical domain Ia (residues 1-16) rests on the surface of the lipid membrane with the hydrophobic face of domain Ia embedded in the membrane bilayer interior. The helix comprised of domain Ib (residues 23-30) and transmembrane domain II (residues 31-52) traverses the bilayer with a tilt angle of approximately 24 degrees . The specific interactions between PLN and lipid membranes may represent an additional regulatory element of its inhibitory function. We propose this hybrid method for the simultaneous determination of structure and topology for membrane proteins with compact folds or proteins whose spatial arrangement is dictated by their specific interactions with lipid bilayers. Structure and topology of monomeric phospholamban in lipid membranes determined by a hybrid solution and solid-state NMR approach.,Traaseth NJ, Shi L, Verardi R, Mullen DG, Barany G, Veglia G Proc Natl Acad Sci U S A. 2009 Jun 23;106(25):10165-70. Epub 2009 Jun 9. PMID:19509339[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences |
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