4pdw: Difference between revisions
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==A benzonitrile analogue inhibits rhinovirus replication== | ==A benzonitrile analogue inhibits rhinovirus replication== | ||
<StructureSection load='4pdw' size='340' side='right' caption='[[4pdw]], [[Resolution|resolution]] 3.00Å' scene=''> | <StructureSection load='4pdw' size='340' side='right'caption='[[4pdw]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4pdw]] is a 4 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4pdw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhinovirus_B Rhinovirus B] and [https://en.wikipedia.org/wiki/Rhinovirus_B14 Rhinovirus B14]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PDW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PDW FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2XK:4-[(4,5-DIMETHOXY-2-NITROPHENYL)ACETYL]BENZONITRILE'>2XK</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>< | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> | ||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2XK:4-[(4,5-DIMETHOXY-2-NITROPHENYL)ACETYL]BENZONITRILE'>2XK</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
<table> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pdw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pdw OCA], [https://pdbe.org/4pdw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pdw RCSB], [https://www.ebi.ac.uk/pdbsum/4pdw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pdw ProSAT]</span></td></tr> | ||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/POLG_HRV14 POLG_HRV14] Capsid proteins VP1, VP2, VP3 and VP4 form a closed capsid enclosing the viral positive strand RNA genome. VP4 lies on the inner surface of the protein shell formed by VP1, VP2 and VP3. All the three latter proteins contain a beta-sheet structure called beta-barrel jelly roll. Together they form an icosahedral capsid (T=3) composed of 60 copies of each VP1, VP2, and VP3, with a diameter of approximately 300 Angstroms. VP1 is situated at the 12 fivefold axes, whereas VP2 and VP3 are located at the quasi-sixfold axes (By similarity). The capsid interacts with human ICAM1 to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin- and caveolin-independent endocytosis. VP0 precursor is a component of immature procapsids (By similarity). Protein 2A is a cysteine protease that is responsible for the cleavage between the P1 and P2 regions. It cleaves the host translation initiation factor EIF4G1, in order to shut down the capped cellular mRNA transcription (By similarity). Protein 2B affects membrane integrity and cause an increase in membrane permeability (By similarity). Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity). Protein 3A, via its hydrophobic domain, serves as membrane anchor (By similarity). Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity). RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity). | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4pdw" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[RNA-directed RNA polymerase|RNA-directed RNA polymerase]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Rhinovirus | [[Category: Rhinovirus B]] | ||
[[Category: Franco | [[Category: Rhinovirus B14]] | ||
[[Category: Froeyen | [[Category: Franco D]] | ||
[[Category: Guerra | [[Category: Froeyen M]] | ||
[[Category: Lacroix | [[Category: Guerra P]] | ||
[[Category: Leyssen | [[Category: Lacroix C]] | ||
[[Category: Neyts | [[Category: Leyssen P]] | ||
[[Category: Querol-Audi | [[Category: Neyts J]] | ||
[[Category: Roche | [[Category: Querol-Audi J]] | ||
[[Category: Terme | [[Category: Roche M]] | ||
[[Category: Vanelle | [[Category: Terme T]] | ||
[[Category: Verdaguer | [[Category: Vanelle P]] | ||
[[Category: Verdaguer N]] | |||