4cr7: Difference between revisions
No edit summary |
No edit summary |
||
| (5 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Crystal structure of the N-acetyl-D-mannosamine dehydrogenase with n-acetylmannosamine== | |||
<StructureSection load='4cr7' size='340' side='right'caption='[[4cr7]], [[Resolution|resolution]] 2.15Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4cr7]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Flavobacterium_sp._141-8 Flavobacterium sp. 141-8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CR7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CR7 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BM3:2-(ACETYLAMINO)-2-DEOXY-ALPHA-D-MANNOPYRANOSE'>BM3</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cr7 OCA], [https://pdbe.org/4cr7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cr7 RCSB], [https://www.ebi.ac.uk/pdbsum/4cr7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cr7 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/DHMA_FLAS1 DHMA_FLAS1] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
N-acetyl-D-mannosamine dehydrogenase (NAMDH) from the soil bacteroidete Flavobacterium sp 141-8 catalyses a rare NAD+-dependent oxidation of N-acetyl-D-mannosamine (ManNAc) into N-acetylmannosamino-lactone, which spontaneously hydrolyses into N-acetylmannosaminic acid. NAMDH belongs to the SDR (short-chain dehydrogenase/reductase) superfamily representing the only NAMDH characterized to date. Thorough functional, stability, site-directed mutagenesis and crystallographic studies have been carried out to better understand the structural and biochemical aspects of this unique enzyme. NAMDH exhibited remarkable alkaline pH optimum (pH 9.4) with a high thermal stability in glycine buffer (Tm = 64 masculineC) and a strict selectivity towards ManNAc and NAD+. Crystal structures of ligand-free and N-acetyl-D-mannosamine and NAD+-bound enzyme forms revealed a compact homotetramer having point 222 symmetry, formed by subunits presenting the characteristic SDR alpha3beta7alpha3 sandwich fold. A highly developed C-terminal tail used as a latch connecting nearby subunits stabilizes the tetramer. A dense network of polar interactions with the substrate including the encasement of its acetamido group in a specific binding pocket and the hydrogen binding of the sugar 4OH atom ensure specificity for N-acetyl-D-mannosamine.The NAMDH-substrate complexes and site-directed mutagenesis studies identify the catalytic tetrad and provide useful traits for identifying new NAMDH sequences. | |||
Crystal structures and functional studies clarify substrate selectivity and catalytic residues for the unique orphan enzyme N-acetyl-D-mannosamine dehydrogenase.,Sola-Carvajal A, Gil-Ortiz F, Garcia-Carmona F, Rubio V, Sanchez-Ferrer A Biochem J. 2014 Jun 27. PMID:24969681<ref>PMID:24969681</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4cr7" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Flavobacterium sp. 141-8]] | |||
[[Category: Large Structures]] | |||
[[Category: Garcia-Carmona F]] | |||
[[Category: Gil-Ortiz F]] | |||
[[Category: Rubio V]] | |||
[[Category: Sanchez-Ferrer A]] | |||
[[Category: Sola-Carvajal A]] | |||