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==Structure of Salmonella typhi type I dehydroquinase irreversibly inhibited with a 1,3,4-trihydroxyciclohexane-1-carboxylic acid derivative== | ==Structure of Salmonella typhi type I dehydroquinase irreversibly inhibited with a 1,3,4-trihydroxyciclohexane-1-carboxylic acid derivative== | ||
<StructureSection load='4clm' size='340' side='right' caption='[[4clm]], [[Resolution|resolution]] 1.40Å' scene=''> | <StructureSection load='4clm' size='340' side='right'caption='[[4clm]], [[Resolution|resolution]] 1.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4clm]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4clm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhi Salmonella enterica subsp. enterica serovar Typhi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CLM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CLM FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene>, <scene name='pdbligand=WPL:(1S,3R,4R,5R)-3-(AMINOMETHYL)-1,4,5-TRIHYDROXYCYCLOHEXANECARBOXYLIC+ACID'>WPL</scene | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene>, <scene name='pdbligand=WPL:(1S,3R,4R,5R)-3-(AMINOMETHYL)-1,4,5-TRIHYDROXYCYCLOHEXANECARBOXYLIC+ACID'>WPL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4clm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4clm OCA], [https://pdbe.org/4clm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4clm RCSB], [https://www.ebi.ac.uk/pdbsum/4clm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4clm ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/AROD_SALTI AROD_SALTI] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 20: | Line 21: | ||
==See Also== | ==See Also== | ||
*[[Dehydroquinase|Dehydroquinase]] | *[[Dehydroquinase 3D structures|Dehydroquinase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Salmonella enterica subsp. enterica serovar Typhi]] | ||
[[Category: Blanco | [[Category: Blanco B]] | ||
[[Category: Gonzalez-Bello | [[Category: Gonzalez-Bello C]] | ||
[[Category: Hawkins | [[Category: Hawkins AR]] | ||
[[Category: Lamb | [[Category: Lamb H]] | ||
[[Category: Lence | [[Category: Lence E]] | ||
[[Category: Llamas-Saiz | [[Category: Llamas-Saiz AL]] | ||
[[Category: Maneiro | [[Category: Maneiro M]] | ||
[[Category: Otero | [[Category: Otero JM]] | ||
[[Category: Peon | [[Category: Peon A]] | ||
[[Category: Poza | [[Category: Poza S]] | ||
[[Category: Sedes A]] | |||
[[Category: Sedes | [[Category: Tizon L]] | ||
[[Category: Tizon | [[Category: Van Raaij MJ]] | ||
[[Category: | |||
Latest revision as of 15:12, 20 December 2023
Structure of Salmonella typhi type I dehydroquinase irreversibly inhibited with a 1,3,4-trihydroxyciclohexane-1-carboxylic acid derivativeStructure of Salmonella typhi type I dehydroquinase irreversibly inhibited with a 1,3,4-trihydroxyciclohexane-1-carboxylic acid derivative
Structural highlights
FunctionPublication Abstract from PubMedThe irreversible inhibition of type I dehydroquinase (DHQ1), the third enzyme of the shikimic acid pathway, is investigated by structural, biochemical and computational studies. Two epoxides, which are mimetics of the natural substrate, were designed as irreversible inhibitors of the DHQ1 enzyme and to study the binding requirements of the linkage to the enzyme. The epoxide with the S configuration caused the covalent modification of the protein whereas no reaction was obtained with its epimer. The first crystal structure of DHQ1 from Salmonella typhi covalently modified by the S epoxide, which is reported at 1.4 A, revealed that the modified ligand is surprisingly covalently attached to the essential Lys170 by the formation of a stable Schiff base. The experimental and molecular dynamics simulation studies reported here highlight the huge importance of the conformation of the C3 carbon of the ligand for covalent linkage to this type of aldolase I enzyme, revealed the key role played by the essential His143 as a Lewis acid in this process and show the need for a neatly closed active site for catalysis. Irreversible covalent modification of type I dehydroquinase with a stable Schiff base.,Tizon L, Maneiro M, Peon A, Otero JM, Lence E, Poza S, van Raaij MJ, Thompson P, Hawkins AR, Gonzalez-Bello C Org Biomol Chem. 2014 Nov 5. PMID:25370445[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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