4ckb: Difference between revisions

<table><tr><td colspan='2'>[[4ckb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CKB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CKB FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>

<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ckb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ckb OCA], [https://pdbe.org/4ckb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ckb RCSB], [https://www.ebi.ac.uk/pdbsum/4ckb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ckb ProSAT]</span></td></tr>

[https://www.uniprot.org/uniprot/MCEL_VACCW MCEL_VACCW] Catalytic subunit of the mRNA capping enzyme which catalyzes three enzymatic reactions: the 5' triphosphate end of the pre-mRNA is hydrolyzed to a diphosphate by RNA 5' triphosphatase; the diphosphate RNA end is capped with GMP by RNA guanylyltransferase and the GpppN cap is methylated by RNA (guanine-N7) methyltransferase. Heterodimeric mRNA capping enzyme catalyzes the linkage of a N7-methyl-guanosine moiety to the first transcribed nucleotide (cap 0 structure), whereas the polymerase associated VP39 is responsible for a second methylation at the 2'-O position of the ribose (cap 1 structure).<ref>PMID:18295814</ref> <ref>PMID:18455214</ref> <ref>PMID:18256245</ref>  The heterodimeric enzyme is also involved in early viral gene transcription termination and intermediate viral gene transcription initiation. Early gene transcription termination requires the termination factor VTF, the DNA-dependent ATPase NPH-I and the Rap94 subunit of the viral RNA polymerase, as well as the presence of a specific termination motif. Binds, together with RAP94, to the termination motif 5'-UUUUUNU-3' in the nascent early mRNA.<ref>PMID:18295814</ref> <ref>PMID:18455214</ref> <ref>PMID:18256245</ref>  

[[Category: Chang J]]

[[Category: Cusack S]]

[[Category: Kyrieleis OJP]]

[[Category: Shuman S]]

[[Category: De la Pena M]]