4cfi: Difference between revisions
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==3D structure of FliC from Burkholderia pseudomallei== | |||
<StructureSection load='4cfi' size='340' side='right'caption='[[4cfi]], [[Resolution|resolution]] 1.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4cfi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei_K96243 Burkholderia pseudomallei K96243]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CFI FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cfi OCA], [https://pdbe.org/4cfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cfi RCSB], [https://www.ebi.ac.uk/pdbsum/4cfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cfi ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/P70944_BURPE P70944_BURPE] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Burkholderia pseudomallei is a Gram-negative bacterium responsible for melioidosis, a serious and often fatal infectious disease that is poorly controlled by existing treatments. Due to its inherent resistance to the major antibiotic classes and its facultative intracellular pathogenicity, an effective vaccine would be extremely desirable, along with appropriate prevention and therapeutic management. One of the main subunit vaccine candidates is flagellin of Burkholderia pseudomallei (FliCBp). Here, we present the high resolution crystal structure of FliCBp and report the synthesis and characterization of three peptides predicted to be both B and T cell FliCBp epitopes, by both structure-based in silico methods, and sequence-based epitope prediction tools. All three epitopes were shown to be immunoreactive against human IgG antibodies and to elicit cytokine production from human peripheral blood mononuclear cells. Furthermore, two of the peptides (F51-69 and F270-288) were found to be dominant immunoreactive epitopes, and their antibodies enhanced the bactericidal activities of purified human neutrophils. The epitopes derived from this study may represent potential melioidosis vaccine components. | |||
Sequence- and Structure-Based Immunoreactive Epitope Discovery for Burkholderia pseudomallei Flagellin.,Nithichanon A, Rinchai D, Gori A, Lassaux P, Peri C, Conchillio-Sole O, Ferrer-Navarro M, Gourlay LJ, Nardini M, Vila J, Daura X, Colombo G, Bolognesi M, Lertmemonkolchai G PLoS Negl Trop Dis. 2015 Jul 29;9(7):e0003917. doi: 10.1371/journal.pntd.0003917., eCollection 2015 Jul. PMID:26222657<ref>PMID:26222657</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4cfi" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Flagellin 3D structures|Flagellin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Burkholderia pseudomallei K96243]] | |||
[[Category: Large Structures]] | |||
[[Category: Bolognesi M]] | |||
[[Category: Colombo G]] | |||
[[Category: Conchillo-Sole O]] | |||
[[Category: Daura X]] | |||
[[Category: Ferrer-Navarro M]] | |||
[[Category: Gourlay LJ]] | |||
[[Category: Lassaux P]] | |||
[[Category: Peri C]] | |||
Latest revision as of 15:09, 20 December 2023
3D structure of FliC from Burkholderia pseudomallei3D structure of FliC from Burkholderia pseudomallei
Structural highlights
FunctionPublication Abstract from PubMedBurkholderia pseudomallei is a Gram-negative bacterium responsible for melioidosis, a serious and often fatal infectious disease that is poorly controlled by existing treatments. Due to its inherent resistance to the major antibiotic classes and its facultative intracellular pathogenicity, an effective vaccine would be extremely desirable, along with appropriate prevention and therapeutic management. One of the main subunit vaccine candidates is flagellin of Burkholderia pseudomallei (FliCBp). Here, we present the high resolution crystal structure of FliCBp and report the synthesis and characterization of three peptides predicted to be both B and T cell FliCBp epitopes, by both structure-based in silico methods, and sequence-based epitope prediction tools. All three epitopes were shown to be immunoreactive against human IgG antibodies and to elicit cytokine production from human peripheral blood mononuclear cells. Furthermore, two of the peptides (F51-69 and F270-288) were found to be dominant immunoreactive epitopes, and their antibodies enhanced the bactericidal activities of purified human neutrophils. The epitopes derived from this study may represent potential melioidosis vaccine components. Sequence- and Structure-Based Immunoreactive Epitope Discovery for Burkholderia pseudomallei Flagellin.,Nithichanon A, Rinchai D, Gori A, Lassaux P, Peri C, Conchillio-Sole O, Ferrer-Navarro M, Gourlay LJ, Nardini M, Vila J, Daura X, Colombo G, Bolognesi M, Lertmemonkolchai G PLoS Negl Trop Dis. 2015 Jul 29;9(7):e0003917. doi: 10.1371/journal.pntd.0003917., eCollection 2015 Jul. PMID:26222657[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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