4c74: Difference between revisions

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-{{STRUCTURE_4c74|  PDB=4c74  |  SCENE=  }}
-===Phenylacetone monooxygenase: Reduced enzyme in complex with APADP===
-{{ABSTRACT_PUBMED_24443704}}
-==Function==
+==Phenylacetone monooxygenase: Reduced enzyme in complex with APADP==
-[[http://www.uniprot.org/uniprot/PAMO_THEFY PAMO_THEFY]] Catalyzes a Baeyer-Villiger oxidation reaction, i.e. the insertion of an oxygen atom into a carbon-carbon bond adjacent to a carbonyl, which converts ketones to esters. Is most efficient with phenylacetone as substrate, leading to the formation of benzyl acetate. Can also oxidize other aromatic ketones (benzylacetone, alpha-methylphenylacetone and 4-hydroxyacetophenone), some aliphatic ketones (dodecan-2-one and bicyclohept-2-en-6-one) and sulfides (e.g. methyl 4-tolylsulfide).
+<StructureSection load='4c74' size='340' side='right'caption='[[4c74]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4c74]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C74 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.97&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=N01:3-ACETYLPYRIDINE+ADENINE+DINUCLEOTIDE'>N01</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c74 OCA], [https://pdbe.org/4c74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c74 RCSB], [https://www.ebi.ac.uk/pdbsum/4c74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c74 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PAMO_THEFY PAMO_THEFY] Catalyzes a Baeyer-Villiger oxidation reaction, i.e. the insertion of an oxygen atom into a carbon-carbon bond adjacent to a carbonyl, which converts ketones to esters. Is most efficient with phenylacetone as substrate, leading to the formation of benzyl acetate. Can also oxidize other aromatic ketones (benzylacetone, alpha-methylphenylacetone and 4-hydroxyacetophenone), some aliphatic ketones (dodecan-2-one and bicyclohept-2-en-6-one) and sulfides (e.g. methyl 4-tolylsulfide).
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+A general question in biochemistry is the interplay between the chemical properties of cofactors and the surrounding protein matrix. Here, the functions of NADP+ and FAD are explored by investigation of a representative monooxygenase reconstituted with chemically-modified cofactor analogues. Like pieces of a jigsaw puzzle, the enzyme active site juxtaposes the flavin and nicotinamide rings, harnessing their H-bonding and steric properties to finely construct an oxygen-reacting center that restrains the flavin-peroxide intermediate in a catalytically-competent orientation. Strikingly, the regio- and stereoselectivities of the reaction are essentially unaffected by cofactor modifications. These observations indicate a remarkable robustness of this complex multi-cofactor active site, which has implications for enzyme design based on cofactor engineering approaches.
-==About this Structure==
+Beyond the Protein Matrix: Probing Cofactor Variants in a Baeyer-Villiger Oxygenation Reaction.,Martinoli C, Dudek HM, Orru R, Edmondson DE, Fraaije MW, Mattevi A ACS Catal. 2013;3(12):3058-3062. PMID:24443704<ref>PMID:24443704</ref>
-[[4c74]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C74 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<ref group="xtra">PMID:024443704</ref><references group="xtra"/><references/>
+</div>
-[[Category: Phenylacetone monooxygenase]]
+<div class="pdbe-citations 4c74" style="background-color:#fffaf0;"></div>
-[[Category: Fraaije, M W.]]
-[[Category: Martinoli, C.]]
+==See Also==
-[[Category: Mattevi, A.]]
+*[[Monooxygenase 3D structures|Monooxygenase 3D structures]]
-[[Category: Baeyer-villiger]]
+== References ==
-[[Category: Cofactor]]
+<references/>
-[[Category: Flavin]]
+__TOC__
-[[Category: Oxidoreductase]]
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Thermobifida fusca]]
+[[Category: Fraaije MW]]
+[[Category: Martinoli C]]
+[[Category: Mattevi A]]