4b9j: Difference between revisions
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==Structure of self-complemented CssA subunit of enterotoxigenic Escherichia coli colonization factor CS6== | |||
<StructureSection load='4b9j' size='340' side='right'caption='[[4b9j]], [[Resolution|resolution]] 2.54Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4b9j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B9J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B9J FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.542Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b9j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b9j OCA], [https://pdbe.org/4b9j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b9j RCSB], [https://www.ebi.ac.uk/pdbsum/4b9j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b9j ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/CSSA1_ECOLX CSSA1_ECOLX] Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Coli surface antigen 6 (CS6) is a widely expressed enterotoxigenic Escherichia coli (ETEC) colonization factor that mediates bacterial attachment to the small intestinal epithelium. CS6 is a polymer of two protein subunits CssA and CssB, which are secreted and assembled on the cell surface via the CssC/CssD chaperone usher (CU) pathway. Here, we present an atomic resolution model for the structure of CS6 based on the results of X-ray crystallographic, spectroscopic and biochemical studies, and suggest a mechanism for CS6-mediated adhesion. We show that the CssA and CssB subunits are assembled alternately in linear fibres by the principle of donor strand complementation. This type of fibre assembly is novel for CU assembled adhesins. We also show that both subunits in the fibre bind to receptors on epithelial cells, and that CssB, but not CssA, specifically recognizes the extracellular matrix protein fibronectin. Taken together, structural and functional results suggest that CS6 is an adhesive organelle of a novel type, a hetero-polyadhesin that is capable of polyvalent attachment to different receptors. | |||
Crystal structure of enterotoxigenic Escherichia coli colonization factor CS6 reveals a novel type of functional assembly.,Roy SP, Rahman MM, Yu XD, Tuittila M, Knight SD, Zavialov AV Mol Microbiol. 2012 Oct 10. doi: 10.1111/mmi.12044. PMID:23046340<ref>PMID:23046340</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4b9j" style="background-color:#fffaf0;"></div> | |||
== | ==See Also== | ||
[[ | *[[Pilin 3D structures|Pilin 3D structures]] | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Knight | [[Category: Large Structures]] | ||
[[Category: Rahman | [[Category: Knight SD]] | ||
[[Category: Roy | [[Category: Rahman MM]] | ||
[[Category: Tuittila | [[Category: Roy SP]] | ||
[[Category: Yu | [[Category: Tuittila M]] | ||
[[Category: Zavialov | [[Category: Yu XD]] | ||
[[Category: Zavialov AV]] | |||
Latest revision as of 14:46, 20 December 2023
Structure of self-complemented CssA subunit of enterotoxigenic Escherichia coli colonization factor CS6Structure of self-complemented CssA subunit of enterotoxigenic Escherichia coli colonization factor CS6
Structural highlights
FunctionCSSA1_ECOLX Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs. Publication Abstract from PubMedColi surface antigen 6 (CS6) is a widely expressed enterotoxigenic Escherichia coli (ETEC) colonization factor that mediates bacterial attachment to the small intestinal epithelium. CS6 is a polymer of two protein subunits CssA and CssB, which are secreted and assembled on the cell surface via the CssC/CssD chaperone usher (CU) pathway. Here, we present an atomic resolution model for the structure of CS6 based on the results of X-ray crystallographic, spectroscopic and biochemical studies, and suggest a mechanism for CS6-mediated adhesion. We show that the CssA and CssB subunits are assembled alternately in linear fibres by the principle of donor strand complementation. This type of fibre assembly is novel for CU assembled adhesins. We also show that both subunits in the fibre bind to receptors on epithelial cells, and that CssB, but not CssA, specifically recognizes the extracellular matrix protein fibronectin. Taken together, structural and functional results suggest that CS6 is an adhesive organelle of a novel type, a hetero-polyadhesin that is capable of polyvalent attachment to different receptors. Crystal structure of enterotoxigenic Escherichia coli colonization factor CS6 reveals a novel type of functional assembly.,Roy SP, Rahman MM, Yu XD, Tuittila M, Knight SD, Zavialov AV Mol Microbiol. 2012 Oct 10. doi: 10.1111/mmi.12044. PMID:23046340[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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