4b5c: Difference between revisions
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== | ==Crystal structure of the peptidoglycan-associated lipoprotein from Burkholderia pseudomallei== | ||
[[4b5c]] is a 3 chain structure with sequence from [ | <StructureSection load='4b5c' size='340' side='right'caption='[[4b5c]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4b5c]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei Burkholderia pseudomallei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B5C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B5C FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b5c OCA], [https://pdbe.org/4b5c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b5c RCSB], [https://www.ebi.ac.uk/pdbsum/4b5c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b5c ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q63RA7_BURPS Q63RA7_BURPS] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We solved the crystal structure of Burkholderia pseudomallei acute phase antigen BPSL2765 in the context of a structural vaccinology study, in the area of melioidosis vaccine development. Based on the structure, we applied a recently developed method for epitope design that combines computational epitope predictions with in vitro mapping experiments and successfully identified a consensus sequence within the antigen that, when engineered as a synthetic peptide, was selectively immunorecognized to the same extent as the recombinant protein in sera from melioidosis-affected subjects. Antibodies raised against the consensus peptide were successfully tested in opsonization bacterial killing experiments and antibody-dependent agglutination tests of B. pseudomallei. Our strategy represents a step in the development of immunodiagnostics, in the production of specific antibodies and in the optimization of antigens for vaccine development, starting from structural and physicochemical principles. | |||
Exploiting the Burkholderia pseudomallei acute phase antigen BPSL2765 for structure-based epitope discovery/design in structural vaccinology.,Gourlay LJ, Peri C, Ferrer-Navarro M, Conchillo-Sole O, Gori A, Rinchai D, Thomas RJ, Champion OL, Michell SL, Kewcharoenwong C, Nithichanon A, Lassaux P, Perletti L, Longhi R, Lertmemongkolchai G, Titball RW, Daura X, Colombo G, Bolognesi M Chem Biol. 2013 Sep 19;20(9):1147-56. doi: 10.1016/j.chembiol.2013.07.010. Epub, 2013 Aug 29. PMID:23993463<ref>PMID:23993463</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4b5c" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Pal|Pal]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Burkholderia pseudomallei]] | [[Category: Burkholderia pseudomallei]] | ||
[[Category: Bolognesi | [[Category: Large Structures]] | ||
[[Category: Colombo | [[Category: Bolognesi M]] | ||
[[Category: Conchillo-Sole | [[Category: Colombo G]] | ||
[[Category: Daura | [[Category: Conchillo-Sole O]] | ||
[[Category: Ferrer-Navarro | [[Category: Daura X]] | ||
[[Category: Gori | [[Category: Ferrer-Navarro M]] | ||
[[Category: Gourlay | [[Category: Gori A]] | ||
[[Category: Lassaux | [[Category: Gourlay LJ]] | ||
[[Category: Lertmemongkolchai | [[Category: Lassaux P]] | ||
[[Category: Longhi | [[Category: Lertmemongkolchai G]] | ||
[[Category: Peri | [[Category: Longhi R]] | ||
[[Category: Rinchai | [[Category: Peri C]] | ||
[[Category: Rinchai D]] | |||
Latest revision as of 14:43, 20 December 2023
Crystal structure of the peptidoglycan-associated lipoprotein from Burkholderia pseudomalleiCrystal structure of the peptidoglycan-associated lipoprotein from Burkholderia pseudomallei
Structural highlights
FunctionPublication Abstract from PubMedWe solved the crystal structure of Burkholderia pseudomallei acute phase antigen BPSL2765 in the context of a structural vaccinology study, in the area of melioidosis vaccine development. Based on the structure, we applied a recently developed method for epitope design that combines computational epitope predictions with in vitro mapping experiments and successfully identified a consensus sequence within the antigen that, when engineered as a synthetic peptide, was selectively immunorecognized to the same extent as the recombinant protein in sera from melioidosis-affected subjects. Antibodies raised against the consensus peptide were successfully tested in opsonization bacterial killing experiments and antibody-dependent agglutination tests of B. pseudomallei. Our strategy represents a step in the development of immunodiagnostics, in the production of specific antibodies and in the optimization of antigens for vaccine development, starting from structural and physicochemical principles. Exploiting the Burkholderia pseudomallei acute phase antigen BPSL2765 for structure-based epitope discovery/design in structural vaccinology.,Gourlay LJ, Peri C, Ferrer-Navarro M, Conchillo-Sole O, Gori A, Rinchai D, Thomas RJ, Champion OL, Michell SL, Kewcharoenwong C, Nithichanon A, Lassaux P, Perletti L, Longhi R, Lertmemongkolchai G, Titball RW, Daura X, Colombo G, Bolognesi M Chem Biol. 2013 Sep 19;20(9):1147-56. doi: 10.1016/j.chembiol.2013.07.010. Epub, 2013 Aug 29. PMID:23993463[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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