4b4q: Difference between revisions
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==Crystal Structure of the lectin domain of F18 fimbrial adhesin FedF in complex with blood group A type 1 hexasaccharide== | |||
<StructureSection load='4b4q' size='340' side='right'caption='[[4b4q]], [[Resolution|resolution]] 2.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4b4q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B4Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B4Q FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b4q OCA], [https://pdbe.org/4b4q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b4q RCSB], [https://www.ebi.ac.uk/pdbsum/4b4q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b4q ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q47212_ECOLX Q47212_ECOLX] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
F18-positive enterotoxigenic and Shiga toxin-producing Escherichia coli are responsible for post-weaning diarrhoea and oedema disease in pigs and lead to severe production losses in the farming industry. F18 fimbriae attach to the small intestine of young piglets by latching onto glycosphingolipids with A/H blood group determinants on type 1 core. We demonstrate the N-terminal domain of the F18 fimbrial subunit FedF to be responsible for ABH-mediated attachment and present its X-ray structure in ligand-free form and bound to A and B type 1 hexaoses. The FedF lectin domain comprises a 10-stranded immunoglobulin-like beta-sandwich. Three linear motives, Q(47) -N(50) , H(88) -S(90) and R(117) -T(119) , form a shallow glycan binding pocket near the tip of the domain that is selective for type 1 core glycans in extended conformation. In addition to the glycan binding pocket, a polybasic loop on the membrane proximal surface of FedF lectin domain is shown to be required for binding to piglet enterocytes. Although dispensable for ABH glycan recognition, the polybasic surface adds binding affinity in the context of the host cell membrane, a mechanism that is proposed to direct ABH-glycan binding to cell-bound glycosphingolipids and could allow bacteria to avoid clearance by secreted glycoproteins. | |||
Structural insight in histo-blood group binding by the F18 fimbrial adhesin FedF.,Moonens K, Bouckaert J, Coddens A, Tran T, Panjikar S, De Kerpel M, Cox E, Remaut H, De Greve H Mol Microbiol. 2012 Jul 19. doi: 10.1111/j.1365-2958.2012.08174.x. PMID:22812428<ref>PMID:22812428</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4b4q" style="background-color:#fffaf0;"></div> | |||
== | ==See Also== | ||
[[ | *[[Adhesin 3D structures|Adhesin 3D structures]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
[[Category: Escherichia coli | </StructureSection> | ||
[[Category: Bouckaert | [[Category: Escherichia coli K-12]] | ||
[[Category: Coddens | [[Category: Large Structures]] | ||
[[Category: Cox | [[Category: Bouckaert J]] | ||
[[Category: Greve | [[Category: Coddens A]] | ||
[[Category: Kerpel | [[Category: Cox E]] | ||
[[Category: Moonens | [[Category: De Greve H]] | ||
[[Category: Panjikar | [[Category: De Kerpel M]] | ||
[[Category: Remaut | [[Category: Moonens K]] | ||
[[Category: Tran | [[Category: Panjikar S]] | ||
[[Category: Remaut H]] | |||
[[Category: Tran T]] | |||
Latest revision as of 14:43, 20 December 2023
Crystal Structure of the lectin domain of F18 fimbrial adhesin FedF in complex with blood group A type 1 hexasaccharideCrystal Structure of the lectin domain of F18 fimbrial adhesin FedF in complex with blood group A type 1 hexasaccharide
Structural highlights
FunctionPublication Abstract from PubMedF18-positive enterotoxigenic and Shiga toxin-producing Escherichia coli are responsible for post-weaning diarrhoea and oedema disease in pigs and lead to severe production losses in the farming industry. F18 fimbriae attach to the small intestine of young piglets by latching onto glycosphingolipids with A/H blood group determinants on type 1 core. We demonstrate the N-terminal domain of the F18 fimbrial subunit FedF to be responsible for ABH-mediated attachment and present its X-ray structure in ligand-free form and bound to A and B type 1 hexaoses. The FedF lectin domain comprises a 10-stranded immunoglobulin-like beta-sandwich. Three linear motives, Q(47) -N(50) , H(88) -S(90) and R(117) -T(119) , form a shallow glycan binding pocket near the tip of the domain that is selective for type 1 core glycans in extended conformation. In addition to the glycan binding pocket, a polybasic loop on the membrane proximal surface of FedF lectin domain is shown to be required for binding to piglet enterocytes. Although dispensable for ABH glycan recognition, the polybasic surface adds binding affinity in the context of the host cell membrane, a mechanism that is proposed to direct ABH-glycan binding to cell-bound glycosphingolipids and could allow bacteria to avoid clearance by secreted glycoproteins. Structural insight in histo-blood group binding by the F18 fimbrial adhesin FedF.,Moonens K, Bouckaert J, Coddens A, Tran T, Panjikar S, De Kerpel M, Cox E, Remaut H, De Greve H Mol Microbiol. 2012 Jul 19. doi: 10.1111/j.1365-2958.2012.08174.x. PMID:22812428[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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