4aom: Difference between revisions
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==MTIP and MyoA complex== | ==MTIP and MyoA complex== | ||
<StructureSection load='4aom' size='340' side='right' caption='[[4aom]], [[Resolution|resolution]] 1.94Å' scene=''> | <StructureSection load='4aom' size='340' side='right'caption='[[4aom]], [[Resolution|resolution]] 1.94Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4aom]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4aom]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AOM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AOM FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.939Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aom OCA], [https://pdbe.org/4aom PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aom RCSB], [https://www.ebi.ac.uk/pdbsum/4aom PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aom ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/Q8I4W8_PLAF7 Q8I4W8_PLAF7] | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Cota | [[Category: Plasmodium falciparum]] | ||
[[Category: Douse | [[Category: Cota E]] | ||
[[Category: Holder | [[Category: Douse CH]] | ||
[[Category: Salgado | [[Category: Holder AA]] | ||
[[Category: Simpson | [[Category: Salgado PS]] | ||
[[Category: Tate | [[Category: Simpson PJ]] | ||
[[Category: Thomas | [[Category: Tate EW]] | ||
[[Category: Thomas JC]] | |||
Latest revision as of 14:31, 20 December 2023
MTIP and MyoA complexMTIP and MyoA complex
Structural highlights
FunctionPublication Abstract from PubMedThe interaction between the C-terminal tail of myosin A (MyoA) and its light chain, myosin A tail domain interacting protein (MTIP), is an essential feature of the conserved molecular machinery required for gliding motility and cell invasion by apicomplexan parasites. Recent data indicate that MTIP Ser-107 and/or Ser-108 are targeted for intracellular phosphorylation. Using an optimized MyoA tail peptide to reconstitute the complex, we show that this region of MTIP is an interaction hotspot using x-ray crystallography and NMR, and S107E and S108E mutants were generated to mimic the effect of phosphorylation. NMR relaxation experiments and other biophysical measurements indicate that the S108E mutation serves to break the tight clamp around the MyoA tail, whereas S107E has a smaller but measurable impact. These data are consistent with physical interactions observed between recombinant MTIP and native MyoA from Plasmodium falciparum lysates. Taken together these data support the notion that the conserved interactions between MTIP and MyoA may be specifically modulated by this post-translational modification. Regulation of the Plasmodium Motor Complex: PHOSPHORYLATION OF MYOSIN A TAIL-INTERACTING PROTEIN (MTIP) LOOSENS ITS GRIP ON MyoA.,Douse CH, Green JL, Salgado PS, Simpson PJ, Thomas JC, Langsley G, Holder AA, Tate EW, Cota E J Biol Chem. 2012 Oct 26;287(44):36968-77. doi: 10.1074/jbc.M112.379842. Epub, 2012 Aug 29. PMID:22932904[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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