4aoc: Difference between revisions
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==crystal structure of BC2L-A Lectin from Burkolderia cenocepacia in complex with methyl-heptoside== | ==crystal structure of BC2L-A Lectin from Burkolderia cenocepacia in complex with methyl-heptoside== | ||
<StructureSection load='4aoc' size='340' side='right' caption='[[4aoc]], [[Resolution|resolution]] 2.70Å' scene=''> | <StructureSection load='4aoc' size='340' side='right'caption='[[4aoc]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4aoc]] is a 5 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4aoc]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_cenocepacia_AU_1054 Burkholderia cenocepacia AU 1054]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AOC FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A1Q:METHYL+L-GLYCERO-ALPHA-D-MANNO-HEPTOPYRANOSIDE'>A1Q</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>< | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> | ||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1Q:METHYL+L-GLYCERO-ALPHA-D-MANNO-HEPTOPYRANOSIDE'>A1Q</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<table> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aoc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aoc OCA], [https://pdbe.org/4aoc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aoc RCSB], [https://www.ebi.ac.uk/pdbsum/4aoc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aoc ProSAT]</span></td></tr> | ||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A0H2Y045_BURCA A0A0H2Y045_BURCA] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4aoc" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Burkholderia cenocepacia]] | [[Category: Burkholderia cenocepacia AU 1054]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Cioci G]] | ||
[[Category: Imberty | [[Category: Imberty A]] | ||
[[Category: Kosma | [[Category: Kosma P]] | ||
[[Category: Lameignere | [[Category: Lameignere E]] | ||
[[Category: Malinovska | [[Category: Malinovska L]] | ||
[[Category: Marchetti | [[Category: Marchetti R]] | ||
[[Category: Molinaro | [[Category: Molinaro A]] | ||
[[Category: Silipo | [[Category: Silipo A]] | ||
[[Category: Wimmerova | [[Category: Wimmerova M]] | ||
[[Category: | [[Category: DeCastro C]] | ||
Latest revision as of 14:31, 20 December 2023
crystal structure of BC2L-A Lectin from Burkolderia cenocepacia in complex with methyl-heptosidecrystal structure of BC2L-A Lectin from Burkolderia cenocepacia in complex with methyl-heptoside
Structural highlights
FunctionPublication Abstract from PubMedBacteria from the Burkholderia cepacia complex (Bcc) cause highly contagious pneumonia among cystic fibrosis (CF) patients. Among them, Burkholderia cenocepacia is one of the most dangerous in the Bcc and is the most frequent cause of morbidity and mortality in CF patients. Indeed, it is responsible of "cepacia syndrome", a deadly exacerbation of infection, that is the main cause of poor outcomes in lung transplantation. B. cenocepacia produces several soluble lectins with specificity for fucosylated and mannosylated glycoconjugates. These lectins are present on the bacterial cell surface and it has been proposed that they bind to lipopolysaccharide epitopes. In this work, we report on the interaction of one B. cenocepacia lectin, BC2L-A, with heptose and other manno configured sugar residues. Saturation Transfer Difference (STD) NMR spectroscopy studies of BC2L-A with different mono- and disaccharides demonstrated the requirement of manno configuration with hydroxyl or glycol group at C6 for the binding process. The crystal structure of BC2L-A complexed with the methyl-heptoside confirmed the location of the carbohydrate ring in the binding site and elucidated the orientation of the glycol tail, in agreement with NMR data. Titration calorimetry performed on monosaccharides, heptose disaccharides and bacterial heptose-containing oligosaccharides and polysaccharides confirmed that bacterial cell wall contains carbohydrate epitopes that can bind to BC2L-A. Additionally, the specific binding of fluorescent BC2L-A lectin on B. cenocepacia bacterial surface was demonstrated by electron microscopy. Burkholderia cenocepacia lectin A binding to heptoses from the bacterial lipopolysaccharide.,Marchetti R, Malinovska L, Lameignere E, Adamova L, de Castro C, Cioci G, Stanetty C, Kosma P, Molinaro A, Wimmerova M, Imberty A, Silipo A Glycobiology. 2012 Jul 3. PMID:22763039[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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