4anr: Difference between revisions

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[[Image:4anr.png|left|200px]]


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==Crystal structure of soluble lytic Transglycosylase SltB1 from Pseudomonas aeruginosa==
The line below this paragraph, containing "STRUCTURE_4anr", creates the "Structure Box" on the page.
<StructureSection load='4anr' size='340' side='right'caption='[[4anr]], [[Resolution|resolution]] 1.84&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[4anr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ANR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ANR FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.84&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
{{STRUCTURE_4anr|  PDB=4anr  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4anr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4anr OCA], [https://pdbe.org/4anr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4anr RCSB], [https://www.ebi.ac.uk/pdbsum/4anr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4anr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9HX24_PSEAE Q9HX24_PSEAE]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In Gram-negative bacteria, the bacterial cell wall biosynthetic mechanism requires the coordinated action of enzymes and structural proteins located in the cytoplasm, within the membrane, and in the periplasm of the cell. Its main component, peptidoglycan (PG), is essential for cell division and wall elongation. Penicillin-binding proteins (PBPs) catalyze the last steps of PG biosynthesis, namely the polymerization of glycan chains and the cross-linking of stem peptides, and can be either monofunctional or bifunctional. Their action is coordinated with that of other enzymes essential for cell-wall biosynthesis, such as lytic transglycosylases (LT). Here, we have studied SltB1, an LT from Pseudomonas aeruginosa, and identified that it forms a complex with PBP2, a monofunctional enzyme, which requires the presence of Ca(2+). In addition, we have solved the structure of SltB1 to a high resolution, and identified that it harbors an EF-hand like motif containing a Ca(2+) ion displaying bipyramidal coordination. These studies provide initial structural details that shed light on the interactions between the PG biosynthesis enzymes in P. aeruginosa.


===Crystal structure of soluble lytic Transglycosylase SltB1 from Pseudomonas aeruginosa===
Calcium-Dependent Complex Formation Between PBP2 and Lytic Transglycosylase SltB1 of Pseudomonas aeruginosa.,Nikolaidis I, Izore T, Job V, Thielens N, Breukink E, Dessen A Microb Drug Resist. 2012 Mar 20. PMID:22432706<ref>PMID:22432706</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4anr" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 22432706 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_22432706}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
[[4anr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ANR OCA].
[[Category: Pseudomonas aeruginosa PAO1]]
 
[[Category: Breukink E]]
==Reference==
[[Category: Dessen A]]
<ref group="xtra">PMID:022432706</ref><references group="xtra"/>
[[Category: Izore T]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Job V]]
[[Category: Breukink, E.]]
[[Category: Nikolaidis I]]
[[Category: Dessen, A.]]
[[Category: Thielens N]]
[[Category: Izore, T.]]
[[Category: Job, V.]]
[[Category: Nikolaidis, I.]]
[[Category: Thielens, N.]]
[[Category: Ef-hand like motif]]
[[Category: Lyase]]
[[Category: Peptidoglycan]]

Latest revision as of 14:31, 20 December 2023

Crystal structure of soluble lytic Transglycosylase SltB1 from Pseudomonas aeruginosaCrystal structure of soluble lytic Transglycosylase SltB1 from Pseudomonas aeruginosa

Structural highlights

4anr is a 1 chain structure with sequence from Pseudomonas aeruginosa PAO1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.84Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9HX24_PSEAE

Publication Abstract from PubMed

In Gram-negative bacteria, the bacterial cell wall biosynthetic mechanism requires the coordinated action of enzymes and structural proteins located in the cytoplasm, within the membrane, and in the periplasm of the cell. Its main component, peptidoglycan (PG), is essential for cell division and wall elongation. Penicillin-binding proteins (PBPs) catalyze the last steps of PG biosynthesis, namely the polymerization of glycan chains and the cross-linking of stem peptides, and can be either monofunctional or bifunctional. Their action is coordinated with that of other enzymes essential for cell-wall biosynthesis, such as lytic transglycosylases (LT). Here, we have studied SltB1, an LT from Pseudomonas aeruginosa, and identified that it forms a complex with PBP2, a monofunctional enzyme, which requires the presence of Ca(2+). In addition, we have solved the structure of SltB1 to a high resolution, and identified that it harbors an EF-hand like motif containing a Ca(2+) ion displaying bipyramidal coordination. These studies provide initial structural details that shed light on the interactions between the PG biosynthesis enzymes in P. aeruginosa.

Calcium-Dependent Complex Formation Between PBP2 and Lytic Transglycosylase SltB1 of Pseudomonas aeruginosa.,Nikolaidis I, Izore T, Job V, Thielens N, Breukink E, Dessen A Microb Drug Resist. 2012 Mar 20. PMID:22432706[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. Nikolaidis I, Izore T, Job V, Thielens N, Breukink E, Dessen A. Calcium-Dependent Complex Formation Between PBP2 and Lytic Transglycosylase SltB1 of Pseudomonas aeruginosa. Microb Drug Resist. 2012 Mar 20. PMID:22432706 doi:10.1089/mdr.2012.0006

4anr, resolution 1.84Å

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