4afy: Difference between revisions
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==Crystal structure of the FimX EAL domain in complex with reaction product pGpG== | ==Crystal structure of the FimX EAL domain in complex with reaction product pGpG== | ||
<StructureSection load='4afy' size='340' side='right' caption='[[4afy]], [[Resolution|resolution]] 2.01Å' scene=''> | <StructureSection load='4afy' size='340' side='right'caption='[[4afy]], [[Resolution|resolution]] 2.01Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4afy]] is a 4 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4afy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AFY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AFY FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.01Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4afy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4afy OCA], [https://pdbe.org/4afy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4afy RCSB], [https://www.ebi.ac.uk/pdbsum/4afy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4afy ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9HUK6_PSEAE Q9HUK6_PSEAE] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 16: | Line 18: | ||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4afy" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa PAO1]] | ||
[[Category: Nonin-Lecomte | [[Category: Synthetic construct]] | ||
[[Category: Rety | [[Category: Nonin-Lecomte S]] | ||
[[Category: Robert-Paganin | [[Category: Rety S]] | ||
[[Category: Robert-Paganin J]] | |||
Latest revision as of 14:26, 20 December 2023
Crystal structure of the FimX EAL domain in complex with reaction product pGpGCrystal structure of the FimX EAL domain in complex with reaction product pGpG
Structural highlights
FunctionPublication Abstract from PubMedFimX is a large multidomain protein containing an EAL domain and involved in twitching motility in Pseudomonas aeruginosa. We present here two crystallographic structures of the EAL domain of FimX (residues 438-686): one of the apo form and the other of a complex with 5'-pGpG, the reaction product of the hydrolysis of c-di-GMP. In both crystal forms, the EAL domains form a dimer delimiting a large cavity encompassing the catalytic pockets. The ligand is trapped in this cavity by its sugar phosphate moiety. We confirmed by NMR that the guanine bases are not involved in the interaction in solution. We solved here the first structure of an EAL domain bound to the reaction product 5'-pGpG. Though isolated FimX EAL domain has a very low catalytic activity, which would not be significant compared to other catalytic EAL domains, the structure with the product of the reaction can provides some hints in the mechanism of hydrolysis of the c-di-GMP by EAL domains. Crystal Structure of an EAL Domain in Complex with Reaction Product 5'-pGpG.,Robert-Paganin J, Nonin-Lecomte S, Rety S PLoS One. 2012;7(12):e52424. doi: 10.1371/journal.pone.0052424. Epub 2012 Dec 20. PMID:23285035[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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