4a7d: Difference between revisions

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-[[Image:4a7d.png|left|200px]]
-<!--
+==X-ray crystal structure of HEWL flash-cooled at high pressure==
-The line below this paragraph, containing "STRUCTURE_4a7d", creates the "Structure Box" on the page.
+<StructureSection load='4a7d' size='340' side='right'caption='[[4a7d]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[4a7d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A7D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A7D FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.497&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-{{STRUCTURE_4a7d|  PDB=4a7d  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a7d OCA], [https://pdbe.org/4a7d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a7d RCSB], [https://www.ebi.ac.uk/pdbsum/4a7d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a7d ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+High-pressure freezing (HPF) is a method which allows sample vitrification without cryoprotectants. In the present work, protein crystals were cooled to cryogenic temperatures at a pressure of 210 MPa. In contrast to other HPF methods published to date in the field of cryocrystallography, this protocol involves rapid sample cooling using a standard HPF device. The fast cooling rates allow HPF of protein crystals directly in their mother liquor without the need for cryoprotectants or external reagents. HPF was first attempted with hen egg-white lysozyme and cubic insulin crystals, yielding good to excellent diffraction quality. Non-cryoprotected crystals of the membrane protein photosystem II have been successfully cryocooled for the first time. This indicates that the presented HPF method is well suited to the vitrification of challenging systems with large unit cells and weak crystal contacts.
-===X-ray crystal structure of HEWL flash-cooled at high pressure===
+Fast high-pressure freezing of protein crystals in their mother liquor.,Burkhardt A, Warmer M, Panneerselvam S, Wagner A, Zouni A, Glockner C, Reimer R, Hohenberg H, Meents A Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Apr 1;68(Pt 4):495-500., Epub 2012 Mar 31. PMID:22505429<ref>PMID:22505429</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 4a7d" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_22505429}}, adds the Publication Abstract to the page
+*[[Lysozyme 3D structures|Lysozyme 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 22505429 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_22505429}}
+__TOC__
+</StructureSection>
-==About this Structure==
-[[4a7d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A7D OCA].
-==Reference==
-<ref group="xtra">PMID:022505429</ref><references group="xtra"/>
 [[Category: Gallus gallus]]
-[[Category: Lysozyme]]
+[[Category: Large Structures]]
-[[Category: Burkhardt, A.]]
+[[Category: Burkhardt A]]
-[[Category: Hohenberg, H.]]
+[[Category: Hohenberg H]]
-[[Category: Meents, A.]]
+[[Category: Meents A]]
-[[Category: Panneerselvam, S.]]
+[[Category: Panneerselvam S]]
-[[Category: Reimer, R.]]
+[[Category: Reimer R]]
-[[Category: Wagner, A.]]
+[[Category: Wagner A]]
-[[Category: Warmer, M.]]
+[[Category: Warmer M]]
-[[Category: High-pressure cooling]]
-[[Category: High-pressure protein crystallography]]
-[[Category: Hydrolase]]