4a4m: Difference between revisions

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-[[Image:4a4m.jpg|left|200px]]
-<!--
+==Crystal structure of the light-activated constitutively active N2C, M257Y,D282C rhodopsin mutant in complex with a peptide resembling the C-terminus of the Galpha-protein subunit (GaCT)==
-The line below this paragraph, containing "STRUCTURE_4a4m", creates the "Structure Box" on the page.
+<StructureSection load='4a4m' size='340' side='right'caption='[[4a4m]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[4a4m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A4M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A4M FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
-{{STRUCTURE_4a4m|  PDB=4a4m  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a4m OCA], [https://pdbe.org/4a4m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a4m RCSB], [https://www.ebi.ac.uk/pdbsum/4a4m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a4m ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/OPSD_BOVIN OPSD_BOVIN] Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal (By similarity).<ref>PMID:16908857</ref> <ref>PMID:17060607</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+G protein-coupled receptors (GPCR) are seven transmembrane helix proteins that couple binding of extracellular ligands to conformational changes and activation of intracellular G proteins, GPCR kinases, and arrestins. Constitutively active mutants are ubiquitously found among GPCRs and increase the inherent basal activity of the receptor, which often correlates with a pathological outcome. Here, we have used the M257Y(6.40) constitutively active mutant of the photoreceptor rhodopsin in combination with the specific binding of a C-terminal fragment from the G protein alpha subunit (GalphaCT) to trap a light activated state for crystallization. The structure of the M257Y/GalphaCT complex contains the agonist all-trans-retinal covalently bound to the native binding pocket and resembles the G protein binding metarhodopsin-II conformation obtained by the natural activation mechanism; i.e., illumination of the prebound chromophore 11-cis-retinal. The structure further suggests a molecular basis for the constitutive activity of 6.40 substitutions and the strong effect of the introduced tyrosine based on specific interactions with Y223(5.58) in helix 5, Y306(7.53) of the NPxxY motif and R135(3.50) of the E(D)RY motif, highly conserved residues of the G protein binding site.
-===Crystal structure of the light-activated constitutively active N2C, M257Y,D282C rhodopsin mutant in complex with a peptide resembling the C-terminus of the Galpha-protein subunit (GaCT)===
+Stabilized G protein binding site in the structure of constitutively active metarhodopsin-II.,Deupi X, Edwards P, Singhal A, Nickle B, Oprian D, Schertler G, Standfuss J Proc Natl Acad Sci U S A. 2012 Jan 3;109(1):119-24. Epub 2011 Dec 23. PMID:22198838<ref>PMID:22198838</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 4a4m" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_22198838}}, adds the Publication Abstract to the page
+*[[Rhodopsin 3D structures|Rhodopsin 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 22198838 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_22198838}}
+__TOC__
+</StructureSection>
-==About this Structure==
-[[4a4m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A4M OCA].
-==Reference==
-<ref group="xtra">PMID:022198838</ref><ref group="xtra">PMID:017825322</ref><ref group="xtra">PMID:021389983</ref><references group="xtra"/>
 [[Category: Bos taurus]]
-[[Category: Deupi, X.]]
+[[Category: Large Structures]]
-[[Category: Edwards, P.]]
+[[Category: Deupi X]]
-[[Category: Nickle, B.]]
+[[Category: Edwards P]]
-[[Category: Oprian, D D.]]
+[[Category: Nickle B]]
-[[Category: Schertler, G F.X.]]
+[[Category: Oprian DD]]
-[[Category: Singhal, A.]]
+[[Category: Schertler GFX]]
-[[Category: Standfuss, J.]]
+[[Category: Singhal A]]
-[[Category: G-protein]]
+[[Category: Standfuss J]]
-[[Category: G-protein-coupled receptor]]
-[[Category: Metarhodopsin-ii]]
-[[Category: Signal tansduction]]
-[[Category: Signaling protein]]
-[[Category: Visual system]]